| Objective:To investigate the effect of low molecular weight heparin?LMWH?on the early vascularization of autogenous fat transplantation in SD rats.Methods:After autogenous fat transplantation,rats were randomly divided into three groups:blank control group,low dose group and high dose group,12 rats in each group.In the blank control group,saline was injected subcutaneously,LMWH 200 IU/kg was injected subcutaneously in the low-dose group,while LMWH 400 IU/kg was injected subcutaneously in the high-dose group,once per 2 days for two weeks.The level of vascular endothelial growth factor?VEGF?in serum was measured by enzyme-linked immunosorbent assay,the survival rate of autogenous fat was calculated by the mass ratio before and after operation,the morphology of fat tissue was observed by HE staining,and the capillary density of fat tissue was calculated by factor VIII immunohistochemical staining.Results:After one week's intervention,serum VEGF levels of blank control group,low-dose group and high-dose group were?18.32±2.48?pg·ml-1,?21.83±2.16?pg·ml-1and?23.67±2.32?pg·ml-1.After two weeks of intervention,levels of VEGF in serum of the three groups were?18.73±2.61?,?28.63±3.18?and?34.09±2.97?pg·ml-1.After two weeks,survival rates of autogenous fat transplantation in the three groups were?64.08±4.87?%,?75.03±4.08?%and?80.52±3.96?%and capillary densities of the three groups were?6.73±1.85?,?10.13±2.08?and?13.67±1.96?per HP.Compared with the blank control group,the difference of the above indexes was statistically significant?P<0.05?,while compared with the high-dose group and the low-dose group,the difference of the above indexes was statistically significant?P<0.05?.Conclusion:Low molecular weight heparin can improve the VEGF level,increase the vascular density and the rate of fat sea keeping,and promote the vascularization ofautogenous fat tissue in early stage. |
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