3D Printed Sodium Alginate / Polylysine/Bioactive Glass Scaffold And Its Osteogenic Properties

Bone is an indispensable part of the human body and plays an important role.However,bone defects due to trauma,tumor resection,aging and other issues have affected people's normal lives,so bone defect repair has received more and more attention.The traditional autogenous bone transplantation and allogeneic bone transplantation for the treatment of bone defects have certain deficiencies,and finding effective repair alternative materials has become an urgent problem in the field of bone repair.With the continuous research on biomaterials,the use of degradable biomaterials to construct tissue engineering scaffolds to achieve bone repair has become the focus of research in this field.When constructing bone repair scaffolds,natural bones need to be bionic in composition and structure to achieve good biocompatibility and osteogenic properties.Sodium alginate and polylysine have good biocompatibility and can effectively promote cell proliferation.58 s bioactive glass has good bioactivity and can activate related osteogenic genes to achieve bone repair.At the same time,the 3D printing molding method can regulate the structure of the material,better bionic the stent,and promote the growth of bone tissue.This paper focuses on the preparation of sodium alginate / polylysine / bioactive glass composite scaffolds,designs on structure and materials,and successfully constructs a tissue engineering scaffold with good bone repair performance.The main research content is divided into the following three parts:First,sodium alginate and polylysine were blended to obtain a uniform slurry,and 3D printing was used to form a sodium alginate / polylysine composite scaffold.Calcium ion,glutaraldehyde and EDC / NHS are used to post-treat the system to explore the most suitable post-treatment method.Taken together,EDC / NHS is more suitable for post-treatment of the system,and it can also be seen that the system has good biocompatibility and is suitable as a material for tissue repair.In addition,compared with pure sodium alginate slurry,sodium alginate / polylysine composite slurry is easier to mix evenly,has better viscoelasticity,and is more suitable for printing.Then,adjust the ratio of sodium alginate and polylysine and treat the scaffolds of the same component with polylysine after the preparation is completed,to prepare eight sets of composite scaffolds of different groups.It can be seen that the post-treatment of polylysine improves the elastic modulus of the scaffold,improves the swelling properties of the hydrogel scaffold and promotes cell proliferation and adhesion,and overall improves the properties of the composite scaffold.In different groups,the ratio of sodium alginate to polylysine has a greater mechanical property when the ratio is 1:1,so the component ratio is selected to be 1:1,and the post-treatment of polylysine is selected for subsequent research.This system has good potential for drug loading and compounding with other materials.Finally,the system was doped with 58 s bioactive glass to control the content of bioglass,and successfully prepared sodium alginate / polylysine / bioglass composite scaffolds with different components.The addition of bioglass improves the mechanical properties and biological activity of the system.At the cell level,proliferation and adhesion are improved,and it has good osteogenic properties.In different groups,when the bioglass content is 10%,it has more excellent properties.