In Vitro Killing Effect Of Anti-EGFR Monoclonal Antibody-modified Gold Nanoparticles On Bladder Cancer T24 Cells Under Near-infrared Irradiation

Objective: To evaluate the growth inhibition and heat killing effects of gold nanoparticles and anti-EGFR monoclonal antibody-modified gold nanoparticles on human bladder cancer T24 cells under near-infrared irradiation.Methods: The gold nanoparticles used in the experiment had a diameter of about 10 nm and an OD value of 2.The gold particle surface with the epidermal growth factor receptor monoclonal antibody modifications.First,the cytotoxicity of gold nanoparticles and anti-EGFR monoclonal antibody modified gold nanorods on human bladder cancer T24 cells was detected,and a safe concentration was selected for subsequent experiments;then the experiments were divided into blank control group,simple irradiation group,nanogold rod treatment group.Four groups of functionalized nano-gold rod treatment groups were used to irradiate bladder cancer T24 cells treated with different powers(3W,6W,9W)and different times(2min,5min,8min)at different times(2min,5min,8min)in different experimental groups.The MTT method was used to detect the survival rate of bladder cancer T24 cells in different experimental groups after different NIR light irradiation conditions,and the temperature change of the cell culture medium in different experimental groups was measured using a thermometer;and using a thermometer to measure the temperature change of the cell culture medium in different experimental groups.At the same time,the cell morphological changes of bladder cancer T24 cells in different experimental groups were observed under an inverted phase contrast microscope.The experimental data was analyzed using SPSS24.0 statistical analysis software,and the paired sample t-test was used for comparative analysis.Results: Gold nanoparticles and anti-EGFR monoclonal antibody-modified gold nanoparticles had no significant toxicity to bladder cancer T24 cells at final concentrations of 15% and 30%.At a final concentration of 45%,there was an inhibitory effect on bladder cancer T24 cells,and at a final concentration of 60%,there was a significant inhibitory effect on bladder cancer T24 cells.After irradiation with near-infrared light at different times and powers,there was a statistically different in vitro killing effect on bladder cancer T24 cells between the simple irradiation group and the nano-gold treatment group,and between the simple irradiation group and the functionalized nano-gold treatment group;when irradiated for 6w8 min,9w5min and 9w8 min,there was a statistically significant in vitro killing effect of bladder cancer T24 cells between the gold nanoparticles treatment group and the functionalized gold nanoparticles treatment group.The temperature of the cell culture medium in the simple irradiation group was between 20-30 ?,and the relative survival rate of the cells was not significantly different from that of the blank control group;the culture medium in the functionalized nano-gold rod treatment group was above 40 ?,and the temperature curve increased significantly,and survived and the cells were significantly reduced.Observe the cell morphological changes under the effect of near infrared light for 6w8 min under the inverted phase contrast microscope: the bladder cancer T24 cells in the simple irradiation group and the blank control group were uniform in size and closely arranged;there were cell suspension,broken cells and other phenomena in the nano-gold rod group and the functionalized nano-gold rod group.Conclusion: Gold nanoparticles near-infrared hyperthermia has a killing effect on bladder cancer T24 cells in vitro,and nano-gold nanoparticles modified by anti-EGFR monoclonal antibodies have a stronger killing effect than that.