| Objective Chronic hepatitis B is a composite liver immune disease caused by hepatitis B virus infection.Liver fibrosis caused by HBV virus in the body is the foundation of many chronic liver diseases.With the development of fibrosis,it is easy to develop into cirrhosis,which has a high mortality rate and seriously threatens the life and health of patients.Therefore,early diagnosis and treatment are very important.The imbalance in the ratio of Th17 cells to Treg cells has been confirmed to be related to the development of many diseases.This study examined the changes in the ratio of Th17/Treg and its related cytokines IL-17/IL-10 in the peripheral blood of patients with chronic hepatitis B during liver fibrosis and the change of Th17/Treg ratio in liver tissue,and we explored the role of Th17/Treg balance in the progress of liver fibrosis to cirrhosis,so as to provide a new idea for clinical rapid diagnosis and treatment in the future.Methods We collected 51 cases of chronic hepatitis B patients from the Affiliated Hospital of Qingdao University from June 2017 to December 2017.According to HE and Masson staining,51 patients were divided into 4 groups according to the degree of liver fibrosis,including 5 patients with stage F1 chronic hepatitis B(F1),10 patients with stage F2 chronic hepatitis B(F2),15 patients with stage F3 chronic hepatitis B(F3),and F4 chronic hepatitis B 21 hepatitis patients(F4)and at the same time,w e selected 10 healthy people as controls(HC).The frequency of Th17 and Treg in peripheral blood was monitored by flow cytometry(FCM),and the levels of IL-17 and IL-10 in serum were analyzed by double-antibody ELISA.Numbers of Th17 and Treg in liver tissues of CHB patients were identified by immunohistochemistry,and analyze the change of Th17/Treg ratio and explore the regularity in the progress of liver fibrosis.Results 1.In peripheral blood,with the progress of fibrosis,the frequency of Th17 and Treg cells both increased,but the ratio showed a decrease first,and then increased to the stage of F3 and F4.The frequency ratio of Th17/Treg cells in the F4 group(2.23 ± 0.67)was statistically significant compared with the other three groups,F1vsF4:P<0.001,F2vsF4:P <0.001,F3vsF4:P<0.001.2.In serum,as fibrosis progressed,the levels of IL17 and IL10 showed an upward trend,and their ratiosthe ratio showed a decrease first,and it increased after the F4 stage.The IL-17/IL-10 ratio in the F4 group(2.23 ± 0.67)was statistically significant compared with the other three groups,F1vsF4:P<0.001,F2vsF4:P <0.001,and F3vsF4:P <0.001.3.In liver tissue,the number of Th17 and Treg cells showed an upward trend,and their ratios showed a downward tendency.The Th17/Treg ratio was statistically significant in the F3 group(1.07 ± 0.86)and the F4 group(0.79 ± 0.28)compared with the F1 group(2.08 ± 1.06),F1vsF3:P= 0.008,F1vsF4:P= 0.001.Compared with F2 group(1.51 ± 0.77)and F4 group(0.79 ± 0.28),the difference in Th17/Treg value was statistically significant,P= 0.011.Conclusion 1.The frequency of Th17 cells and Treg cells in the peripheral blood,the level of IL-17 and IL-10 cytokines,and the number of Th17 cells and Treg cells in the liver tissue all increased with the progress of liver fibrosis,and the corresponding ratio also changed,which proved that there was an imbalance of Th17/Treg in the progress of liver fibrosis.2.The imbalance of Th17/Treg is an important consideration to promote the progress of liver fibrosis.The detection of Th17/Treg ratio can reflect the development of liver fibrosis,which has a certain clinical value. |
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