Differential Expression Of Sp1 In Lesionsof Psoriasis Vulgaris And Study On Proliferation Of Keratinocytes

Background As a chronic skin disease that plagues the majority of patients,psoriasis has caused huge harm to patients' lives and psychology for a long time.It has a certain degree of genetic characteristics,which is characterized by excessive epidermal proliferation and the signaling mechanism that causes psoriasis.It is not clear yet.Psoriasis vulgaris(PV)is the most widely used among them.The clinical lethal rate is slightly lower than pustular psoriasis,erythrodermic psoriasis,and articular psoriasis,but the area of epidermal damage It is still very extensive.The main clinical manifestation of psoriasis vulgaris is skin damage,and the severe itching and incomplete appearance produced by the psoriasis patients are deeply troubled.57.3% of psoriasis is moderate to severe.Large areas of the patient's skin desquamation and erythema will cause others to feel nausea and fear.Many patients are isolated from this,resulting in inferiority complex,and even affect their normal life such as spouse marriage,employment and livelihood,34 % Of patients have a tendency to depression and suicide[1],which has a huge impact on the quality of life of patients.The histopathological manifestations of patients with psoriasis have obvious characteristics.The lesions from the top to the bottom include significant epidermal cell hyperplasia,dermal inflammation spread downward,and neoplastic capillary diffusion.Its pathogenesis is extremely complex,and the etiology currently understood mainly covers all aspects of genetics and environment,immunity and infection [1].At present,it is generally believed that excessive proliferation of keratinocytes(KC)under immune-mediated effects is one of the important pathological features of psoriasis.The transcription factor Spl can specifically recognize and bind to the GC-rich region in DNA,and its structural expression is in the skin,breast,uterus,gastrointestinal tract and other parts of the human body.Because Sp1 can be combined with GC sequence-enriched sites,many housekeeping genes and human tissue-specific genes have this structure,so they are all affected by it and thus participate in physiological processes such as cell development and metabolism.This study further explored the influence of Sp1 on the proliferation of psoriatic keratinocytes by studying the correlation between Sp1 and psoriatic lesions and the effect of Sp1 on keratinocytes.Provides a preliminary research basis for the molecular pathogenesis of Sp1 on the occurrence and development of psoriasis.Objective In this study,genetic experimental methods such as immunohistochemical staining(IHC),Real-time quantitative polymerase chain reaction(RT-q PCR),and western blot(WB)were used in skin tissues and epidermal keratinocytes of psoriasis and normal controls.The differential expression of the transcription factor Sp1 was compared.Gene interference technology was used to verify whether the function of epidermal keratinocytes was regulated by Sp1,and the correlation between transcription factor Sp1 and KC was preliminarily discussed.Method From August 2018 to May 2019,the research group received 54 patients with psoriasis outpatients from the dermatology clinic and healthy subjects from the aesthetic department at the Guangdong Dermatology Hospital,respectively.For healthy skin tissues,molecular experiments are conducted according to the conditions corresponding to gender and age,and molecular genetic tests such as immunohistochemical staining(Immunohistochemistry,IHC),Real-time quantitative polymerase chain reaction(RT-q PCR),and Western blot methods,Detect the expression of Sp1 in the skin tissue at the m RNA and protein levels of the pathogenic group and the control group;adopt Gene knock-down to treat Sp1 in Hacat cells to silence their expression,and then detect the growth of the cells Development and other functions to verify the effect of Sp1 on KC function.Results Immunohistochemical staining showed that the expression of transcription factor Sp1 was higher in the psoriasis group than in the normal control group.The psoriasis group was widely expressed in the nuclei of the epidermis,mainly in the spinous layer and basal layer.By calculating the average optical density value,the two groups were statistically analyzed,and the results were statistically significant.(n = 24,p = 0.0127)Molecular biology studies have shown that the psoriatic group in the skin tissue has a higher expression level at the m RNA level and protein level than the healthy control group,and the difference is statistically significant.The difference was statistically significant(RT-q PCR: n = 25,p = 0.0057)(Western blot: n = 56,p<0.0001).Pearson correlation coefficient analysis showed that there was a linear positive correlation between the PASI score and the expression level of Sp1 at the m RNA level,with a relatively large degree of correlation.(R = 0.9173,p <0.0001)Therefore,the severity of psoriasis vulgaris is positively correlated with the expression of Sp1.In terms of cell-level studies,Hacat cells after Sp1 gene knockdown treatment were subjected to cck-8 counting and absolute cell counting.The cells in the knockdown group grew slowly and the number of cells decreased,suggesting that Sp1 can promote keratinocyte proliferation.Conclusions The transcription factor Sp1 is correlated with the pathogenesis of psoriasis vulgaris;the increased expression level of Sp1 in the lesions of patients with psoriasis vulgaris can promote the proliferation of keratinocytes,for further study Sp1 participates in the occurrence and development of psoriasis Provide preliminary research work.