Study On Anti-inflammatory And Bacteriostatic Spectrum-effect Relationship Of Siraitia Grosvenorii In Guizhou

Objective:The main objective of the thesis is to establish the anti-inflammatory and antibacterial spectrum-effect relationship model of Siraitia grosvenorii,and apply it to analysing of the difference of pharmacodynamic components of different polar extracts of Siraitia grosvenorii.Methods:(1)The RAW 264.7 cell inflammation model of mice induced by lipopolysaccharide(LPS)was established,and the anti-inflammatory activity of extracts from different polar parts of Siraitia grosvenorii was studied with the release levels of NO,IL-6 and TNF-? as indicatorsin in vitro.(2)Using xylene-induced mouse ear swelling model to study the effects of extracts from different polar parts of Siraitia grosvenorii on anti-inflammatory activity in vivo.(3)The filter paper method was used to study the antibacterial activity of extracts from different polar parts of Siraitia grosvenorii based on the size of the bacteriostatic circle.(4)By high performance liquid chromatography,the chromatographic conditions of the extracts of different polar parts of Siraitia grosvenorii were investigated,and the fingerprints of the extracts of different polar parts of Siraitia grosvenorii were established.(5)Based on the grey correlation and partial least squares regression analysis,the anti-inflammatory and bacteriostatic spectrum effect relationship model of Siraitia grosvenorii was established.Results:(1)The extracts from the five polar sites could inhibit the release of NO,IL-6 and TNF-? without affecting the cell survival rate,and the inhibitory effect was concentration-dependent.(2)The ethyl acetate site extract the inhibitory effect is stronger than the extracts of other four polar parts.The extracts of five polar parts of Siraitia grosvenorii had an inhibitory effect on mouse ear swelling in a dose-dependent manner.(3)The bacteriostatic results showed that all the five polar parts of Siraitia grosvenorii had different degree of bacteriostatic effect,among which the inhibition effect onEscherichia coli was the most significant.For enterococcus faecalis,only the extracts of water,methanol,ethanol and n-butanol had inhibitory effect.For Pseudomonas aeruginosa,Staphylococcus aureus and Proteus,only three extraction sites had inhibitory effect on the extract of Siraitia grosvenorii.While for Bacillus subtilis,the inhibitory effect was the worst,and only n-butanol and ethyl acetate had inhibitory effect on extracts of Siraitia grosvenori.(4)The fingerprints of extracts of five polar parts of Siraitia grosvenorii were established,nine common peaks were identified,and peaks 8 and 9 were successfully identified,which were 11-O Mogroside V and Mogroside V.(5)The results of gray correlation analysis and partial least squares analysis showed that the extracts of different polar parts of Siraitia grosvenorii's achieved the anti-inflammatory and bacteriostatic activity through composite components,and the main pharmacodynamic contribution peak of Siraitia grosvenorii's anti-inflammatory and antibacterial activity was initially confirmed.Conclusion:Based on the study of bacteriostatic and anti-inflammatory activity in vitro of different polar extraction parts of Siraitia grosvenorii,the spectrum-effect relationship model of anti-inflammatory and bacteriostatic of Siraitia grosvenorii was established by using gray correlation degree and partial least squares analysis,and the main pharmacodynamic component peaks of anti-inflammatory and bacteriostatic activity of Siraitia grosvenorii were determined.This study effectively analyzes the anti-inflammatory and antibacterial activity of Siraitia grosvenorii,and reveals the material basis of the anti-inflammatory and bacteriostatic activity of Siraitia grosvenorii to a certain extent.