Scanning Electron Microscope Observation Of The Effect Of Laser Combined With Potassium Fluoride-formaldehyde Resin On Closing Dentinal Tubules

Objective:The sealing effect of dentin tubule and anti-brushing ability of different desensitization methods were observed by scanning electron microscope.evaluateing zhe effect of potassium fluoride resin desensitizer,laser and laser combined with potassium fluoride phenolic resin desensitizer.So that providing experimental basis and theoretical support for the clinical application of laser combined with fluoropotassium phenolic resin desensitizer.Methods:48 caries free third molars were selected and prepared into 3.0 mm×3.0 mm×2.5 mm dentin blocks.They were randomly divided into four groups according to different desensitization methods.Group A: control group;Group B: potassium fluoride phenolic resin desensitizer group;Group C :laser group;Group D : laser combined with fluoropolyphenolic resin desensitizer group.In group B/C/D,6 samples were randomly selected as immediate group and the remaining 6 samples were selected as wear group for the brushing experiment.Half of the dentin specimens were randomly selected to be split in each group to observe the longitudinal profile.The surface closure of the dentinal tubules of the blank group and the test group was observed by scanning electron microscope.The morphological analysis was performed about the representative areas to observe the closure depth of dentin tubules in longitudinal profile.Using SPSS 24.0analyze all date.Results:1.Group B,group C and group D could effectively block the dentin surface(P <0.05).2.In pair wise comparison,there was no statistical difference in the depth of closure between the b/d groups(P? 0.05),however,both are deeper than group C(P <0.05).3.After brushing tooth,The differences among the three groups in sealing dentin tubuleorifice were significant(P<0.05).Group D was better than group B and group C,group B was better than group C(P <0.05).4.After brushing tooth,as the depth of longitudinal section,group B and Group d were deeper than group C(P<0.05).Conclusion:1.Fluorine potassium phenolic resin desensitizer,laser and the combination of laser and fluorine potassium phenolic resin desensitizer can effective seal dentin surface.2.The effect of the combination of laser and potassium fluoro potassium phenolic resin desensitizing on sealing dentinal tubules is better and more durable than that of desensitizing agent or laser alone.The depth of laser-closed dentinal tubules is small,and the ability of mechanical brushing is poor.