The Expression And Significance Of Krüppel-Like Factor 10 In Urothelial Carcinoma Of Bladder

Objective:Bladder cancer is one of the most common malignant tumors at present.By studying the expression of Krüppel like factor 10(klf10)in normal bladder mucosa and bladder urothelial carcinoma with different clinicopathological factors,this paper reveals the relationship between klf10 and the occurrence,development and clinicopathological factors of bladder urothelial carcinoma,so as to provide theoretical basis for future treatment of bladder urothelial carcinoma.Methods:Screening samples: 50 cases of bladder urothelial carcinoma and 30 cases of normal bladder mucosa adjacent to the cancer were screened.The tissue samples were collected from the inpatients in the Department of Urology of Qingdao Municipal Hospital from May 2018 to October 2019.According to the WHO2004 grading standard of bladder cancer,There were 33 cases of Ta?T1staging and 17 cases of T2?T4 staging,31 cases of high-grade tumors,19 cases of low-grade tumors,34 male patients and 16 female patients,aged from 38 to80 years old,with an average age of 56.0years.Samples were taken and preserved: all the cases included in this study were first-time surgical patients who had not received special treatment(such as radiotherapy,chemotherapy or immunotherapy)before operation.The specimens were fixed with 10% formaldehyde for immunohistochemical detection,and the samples were treated by solid paraffin embedding method,and the tissue was sectioned with a thickness of 4 ?m.The tissue samples of immunoblotting test were collected: immediately after surgical resection,the tissue samples were put into the sterilized Eppendof tube under sterile conditions and cryopreserved in the refrigerator of-80 °C.Study methods: the immunohistochemical expression of KLF10 in bladder urothelial carcinoma and normal bladder mucosa was analyzed,and the difference was analyzed quantitatively by western blotting imaging analysis system.Results:Immunohistochemical results showed that KLF10 was expressed in bladder urothelial carcinoma and normal bladder mucosa in varying degrees.In most cases,KLF10 was diffusely expressed in the cytoplasm,diffusely distributed and yellowish-brown granules,and it could be seen that some cellswere also distributed in the nucleus.The positive expression rate of KLF10 in bladder urothelial carcinoma was 48.0%,and the positive expression rate in normal bladder mucosa was 96.7%.The expression of KLF10 in bladder urothelial carcinoma was significantly lower than that in normal bladder mucosa.The expression of KLF10 in non-invasive bladder urothelial carcinoma was significantly higher than that in invasive bladder cancer.The expression of KLF10 in high-grade bladder cancer was significantly lower than that in low-grade bladder urothelial tissue.There was no significant difference in the expression of KLF10 in bladder urothelial carcinoma among different sex,age,tumor size and other factors.Western blotting quantitative analysis showed that in 60 cases of bladder urothelial carcinoma and normal bladder mucosa,the expression intensity of KLF10 in normal bladder mucosa was higher,KLF10 expression was found in 23 cases of bladder urothelial carcinoma and no expression was found in 7 cases.The electrophoretic results of normal bladder mucosa and urothelial carcinoma are shown in figure 2.The analysis of the ratio of KLF10 to ?-actin band density showed that the expression of KLF10 in bladder urothelial carcinoma was significantly lower than that in matched normal bladder tissues,and the difference was statistically significant.(table 1),which is consistent with the results of immunohistochemistry,indicating that KLF10 may play an inhibitory role in the occurrence and development of bladder urothelial carcinoma.The sample size of this study is small,so Western Blotting has not been used to study the expression and comparison of KLF10 among different clinicopathological factors.Conclusion:1.The positive expression rate of KLF10 in bladder urothelial carcinoma was significantly lower than that in normal bladder mucosa,suggesting that KLF10 may be involved in the occurrence and development of bladder cancer.2.The expression of KLF10 was related to the staging and grading of bladder urothelial carcinoma: the expression in high-grade urothelial carcinoma was lower than that in low-grade urothelial carcinoma,and the expression in non-muscular invasive carcinoma was higher than that in muscular invasive carcinoma.There was no significant correlation between the expression of KLF10 and sex,age and tumor size.It is suggested that the loss of expression of KLF10 protein is closely related to the occurrence and development of bladder cancer,and may become a clinical diagnostic marker and a possible therapeutic target for bladder cancer,and is expected to be helpful in predicting the prognosis of bladder cancer.