Mechanism Of Vitamin D Receptor Modulating Wnt/?-Catenin Signaling Pathway In The Occurrence And Development Of Prostate Cancer

Objectives This study by observing the different status of the vitamin D receptor for the influence of prostate cancer cells malignant phenotype and Wnt/?-catenin signaling pathway expression in various indicators,from the level of cell research vitamin D receptor,Wnt/?-catenin signaling pathways involved in prostate cancer development mechanism.Methods Building system silence VDR gene recombinant plasmid vector.In combination with two package plasmids ps PAX2 and p MD2.G,the PEI mediated three-plasmid system was used to transfect 293 T cells,and the package silenced lentivirus expressing VDR was used to construct a cell model that silenced VDR expression and expressed random sequence.Grouping: DU145 cell group,random sequence control group,silencing VDR genome;The changes of cell migration,apoptosis and the content of-catenin were detected by scratch test and Western-blot.Results 1.Successfully constructed the DU145 model of prostate cancer cells silencing VDR and the control cell model with random sequence.2.silencing the VDR gene promoted the migration of DU145 cells: In 24 h after observation and calculate the scratches found that system silence VDR genome(0.58 ± 0.07)in cell repair,repair random sequence control group compared with(0.18 ± 0.03),DU145 cell repair group compared with(0.21 ± 0.02),the cell migration ability of silencing VDR genome was significantly higher than that of the DU145 cell group and the random sequence control group,and the repair ratio was greater than that of the DU145 cell group and the random sequence control group(P<0.01).DU145 cell group and the proportion of random sequence control cell repair and migration ability was no significant difference(P>0.05).3.Silencing VDR gene inhibited the apoptosis of DU145 cells: the relative bax expression in each group was:(2.12±0.26).Random sequence control group(1.91±0.34);Silence VDR genome(0.72± 0.10).The results showed that the expression of bax protein in silencing VDR genome was significantly down-regulated with significant difference(P<0.01),DU145 cell group and random sequence control group no significant difference.This indicated that silencing VDR inhibited the apoptosis of DU145 cells.4.The silencing of VDR gene promoted the expression of DU145 cells with respect to the relative expression of ?-catenin in each group: DU145 cells(1.02±0.23);Random sequence control group(1.14±0.21);Silence VDR genome(1.96± 0.31).The results showed that the expression of?-catenin protein in the silent VDR genome was significantly up-regulated with significant differences(P<0.01),DU145 cell group and random sequence control group no significant difference.This indicated that the silencing of VDR gene promoted the expression of ?-catenin in DU145 cells.Conclusions 1.Silencing vitamin D receptor can promote DU145 cell migration and inhibit DU145 cell apoptosis.2.Silencing the vitamin D receptor is involved in the development of prostate cancer by promoting the expression of ?-catenin protein and thereby regulating the Wnt/?-catenin signaling pathway.Figure 6;Table 1;Reference 1992.