Effect Of Intraosseous Infusion On Immune Function In Mice With Traumatic Hemorrhagic Shock

Objective The effects of intraosseous infusion replacement on the immune status of traumatic hemorrhagic mice and its mechanism were explored by detecting changes in the number of bone marrow and kidney immune cells and the number of regulatory dendritic cells(CD11c^-CD45RB^highDCs)in bone marrow and kidney.Methods C57BL/6 mice were initially divided into sepsis model group,trauma hemorrhagic shock+sepsis model group,trauma hemorrhagic shock+sepsis model+25 ?L/g normal saline group and trauma hemorrhagic shock+sepsis model+50 ?L/g normal saline group.The mortality rates of the above groups were observed respectively.The mice in the above experimental group were sacrificed on days 0,3,7 and 14 respectively,and bone marrow and kidney tissues were taken.Finally,the obtained bone marrow and kidney tissues were ground,centrifuged,resuspended,and cells were counted.Flow cytometry was used to determine changes in dendritic cell phenotypes and to determine changes in the number of CD11c^-CD45RB^highDCs.At the three time points before traumatic hemorrhagic shock,10 minutes after traumatic hemorrhagic shock and after bone marrow rehydration,blood was collected by eyeball extraction and centrifugation to obtain plasma,and the changes in plasma concentrations of TNF-? and IL-10 were determined by enzyme-linked immunosorbent assay?ELISA?.Immune cells and CD11c^-CD45RB^highDCs from bone marrow and kidney were collected on days 0 and 7,and then intramuscularly injected into sepsis mice with doses of bone marrow cells?10⁶ and 10⁷?,kidney cells?106 and 10⁷?,bone marrow CD11c^-CD45RB^highDCs?10⁵ and 5×10⁵?and kidney CD11c^-CD45RB^highDCs?10⁵ and 2×10⁵?,respectively,to observe the changes in mortality.Results 1 Intraosseous infusion can significantly reduce the mortality of mice with traumatic hemorrhagic shock and sepsis?P<0.05?.Traumatic hemorrhagic shock can also reduce the mortality of septic mice,but it is not obvious compared with the group receiving intraosseous infusion replacement?P<0.05?.2 By increasing the amount of TNF-?,intraosseous infusion can promote the proliferation and differentiation of bone marrow and accelerate the migration of immune cells to peripheral organs.3 After the intraosseous infusion treatment was given,the immune cells of the bone marrow and kidney and CD11c^-CD45RB^highDCs in the bone marrow and kidney were significantly increased in mice,and the range of increase increased with the increase of fluid supplementation in a certain range?P<0.05?.4 The reinfusion of CD11c^-CD45RB^highDCs can reduce the mortality of sepsis mice more than the same dose of bone marrow and immune cells in the kidney?P<0.05?.Conclusion Intraosseous infusion can rapidly replenish the blood volume of the body,promote the proliferation and differentiation of bone marrow into CD11c^-CD45RB^highDCs and enhance the protective effect of innate immunity activated by traumatic hemorrhagic shock.It is because of the proliferation of CD11c^-CD45RB^highDCs and the migration and aggregation of CD11c^-CD45RB^highDCs into underperfused kidneys that they effectively prevent the occurrence of complications such as Sepsis and AKI and reduce the mortality in mice with traumatic hemorrhagic shock.Figure 8;Table 1;Reference 231...