Correlation Between Syndrome Elements And Intestinal Microorganisms In AMCI Patients With Kidney Deficiency And Phlegm Stasis Syndrome

Objective: To investigate the characteristics of intestinal microbiota in patients with amnestic mild cognitive impairment(a MCI)by using 16 s r RNA gene sequencing technology,and to detect the types and abundance of intestinal microbes in a MCI patients with kidney deficiency and phlegm and stasis syndrome.Correlation between cognitive function and intestinal microbes in patients with a MCI and cognitively normal elderly,and analysis of changes in intestinal microbiota abundance between different syndrome elements(kidney deficiency,phlegm,blood stasis),in order to explore the intestinal microbes in a MCI The role of the pathogenesis provides ideas.Methods: Twenty-four patients with a MCI aged 65-85 years old with a syndrome of kidney deficiency,phlegm and blood stasis,and 17 normal cognitive elders were selected from 65-85 years old of Emeishan Civil Administration and Welfare Institute in Sichuan Province.The simple mental state checklist(MMSE)and Montreal cognitive assessment were used.Scale(Mo CA),Alzheimer's disease rating scale cognitive part scale(ADAS-cog),clinical dementia rating scale(CDR),daily living ability scale(ADL),Hachiski ischemia scale(HIS),Hamilton Depression Scale(HAMD),Hamilton Anxiety Assessment Scale(HAMA)for neuropsychological assessment;16s r RNA gene sequencing technology was used to extract DNA from stool samples for PCR amplification,and sequencing was performed on the Illumina Hi Seq 2500 platform.Sample sequence of a MCI patients with kidney deficiency,phlegm and stasis syndromes and cognitively normal elderly were analyzed for intestinal microbiota abundance,biodiversity and significant differences,and analysis of TCM syndrome factors(kidney deficiency,phlegm,blood stasis),cognition Correlation between functional impairment and gut microbes.Result: 1.General information: The male and female ratios in the a MCI group were 29.2% and 70.8%;the normal cognitive control group was 29.4% and 70.6%.The average age of the a MCI group and the normal cognitive control group were 79.21 ± 4.31 years and 78.82 ± 5.43 years,respectively,and those with type 2 diabetes were 20.8% and 29.4%.The education level of the a MCI group was concentrated in primary school(20.8%),junior high school(37.5%),and the education level of normal cognitive control group was concentrated in junior high school,high school(23.5%),and undergraduate(29.4%).2.Scale evaluation: The two groups had significant differences in the scores of the ADAS-cog and Mo CA scales(P <0.001),and the differences were reflected in the scores of the MMSE scale(P <0.05).In the CDR,ADL,HIS,HAMD,There was no significant difference in HAMA scores(P?0.05).3.Intestinal microorganisms:(1)Diversity: A total of 2,575,137 high-quality 16 S r RNA gene sequences(62,808.22 per sample)were obtained,and the number of Effective tags accounted for more than 85% of the number of raw tags.There was no significant difference in the ? diversity index between the two groups(P> 0.05),but PCA analysis in the ? diversity indicated that there was a significant difference between the two groups(P <0.01).(2)Abundance: The two groups are mainly composed of Bacteroides,Pseudomonas,and Proteobacteria at the level of the phylum(P> 0.05);at the level of the family,there are aerobacteriaceae and high-temperature actinomycetes.,Mycobacteriaceae,Rhizobactaceae,Propionibacteriaceae,Microbacteriaceae,Rhodobacteraceae,Sphingomonasceae,vadin BE97,Family-I,etc.showed differences(P <0.01);Aeromonas,Prevotella,Mitsuoka,Dysgonomonas,Pseudomonas,Lentibacterium,Ezakiella,Vibrio butyricum,Heissococcus,Cloppenia,Weisserella,Rhizobium,Stabilizer,Propionibacterium,Paracoccus,Sphingomonas,Howardella,RIKEN_RC9_gut_group,Rumen bacteria,Prochlorococcus,etc.(P <0.01).(3)Correlation: Corynebacterium,Rhodobacteraceae,Desulfovibrioideae,Trichospiraceae,Oxalobactaceae,uncultured_bacterium_p_Saccharibacteria and Kidney Deficiency Syndrome have a significant negative correlation(P <0.01);Bifidobacterium There was a positive correlation between the phlegm and phlegm syndrome scores(P <0.05);the flavinaceae,Leuconostocaceae,and Pseudomonasceae correlated with the blood stasis syndrome scores(P <0.05);Firmicutes)and MOCA scale scores have a negative correlation(P <0.05);Enterobacteriaceae and MMSE scale scores have a significant negative correlation(P <0.01).Patients with type 2 diabetes a MCI also had intestinal Family_XIII_UCG-001,Fibriobacterium,Rumenococcus_UCG-003 and the syndrome elements of kidney deficiency and blood stasis(P <0.05).Conclusion: Compared with the cognitively normal elderly,a MCI patients with kidney deficiency,phlegm and stasis syndromes have significantly different intestinal microbiome diversity and relative abundance levels;the elements of kidney deficiency syndromes are associated with multiple intestinal microorganism manifestations.