The PGF_2?/PGE₂ Balancing Homeostasis Characteristics Study Of Mild Moxibustionin Improving Uterine Microcirculation Of Primary Dysmenorrhea Rats

Objective:This study takes Primary dysmenorrhea as the disease model and clean female SD rats as the research object.On the basis of observing the effect of mild moxibustion on regulating the uterine microcirculation state of PD model rats,it further explores whether the effect of mild moxibustion on the uterine microcirculation state is related to the regulation of PGF_2?/PGE₂ balance homeostasis,so as to explore the function characteristics of mild moxibustion improving the uterine microcirculation state of PD.The clinical application of Moxibustion in the treatment of Primary dysmenorrhea provides more scientific basis.Method:Forty clean female SD rats were domesticated adaptively for 7 days and screened the vagina for 7 days.According to the random number table,they were divided into 4groups,10 rats in each group,namely group A?blank group?,group B?blank with moxibustion group?,group C?PD model group?and group D?moxibustion treatment group?.Group C and group D were induced by estradiol benzoate combined with oxytocin,group A and group B were subcutaneously injected with normal saline of equal volume for a total of 10 days.At the end of modeling,the writhing reaction was observed within 20 minutes.Moxibustion on Sanyinjiao and Guanyuan points for 20minutes.At the end of the treatment,the diameter of microvasculature was observed in vivo.Carotid blood was collected and hemorheological indexes were measured.The contents of PGF_2?and PGE₂ in uterus were measured by enzyme-linked immunosorbent assay.Result:1. Torsion ReactionIn addition to the group A and group B,both group C and group D showed writhing response.Compared with group C,the latent period of writhing body in group A was prolonged,and the number of writhing body was decreased,the difference between two groups was no significant?p>0.05?.2. HemorheologyWhole blood viscosity:compared with group A,the whole blood viscosity of group C under all shear rates decreased significantly?p<0.01?;the whole blood viscosity of group D under all shear rates decreased significantly,the difference was statistically significant?p<0.01?;the whole blood viscosity of group B under all shear rates decreased,the difference was not statistically significant?p>0.05?;compared with group B,the whole blood viscosity of the treatment group was significantly reduced under all shear rates,with no statistical significance?p>0.05?;compared with group C,the whole blood viscosity of group D under all shear rates was increased,with no statistical significance?p>0.05?.Plasma viscosity:compared with group A,the plasma viscosity of group D increased,the difference was statistically significant?p<0.05?;the plasma viscosity of the group B decreased,the difference was not statistically significant?p>0.05?;the plasma viscosity of group C decreased,the difference was not statistically significant?p>0.05?;compared with the group B,the plasma viscosity of the treatment group decreased,the difference was not statistically significant?p>0.05?;Compared with group C,the plasma viscosity of group D decreased,the difference was not statistically significant?p>0.05?.Erythrocyte aggregation index:compared with group A,the erythrocyte aggregation index in group C decreased,with statistical significance?p<0.01?;the erythrocyte aggregation index in group D decreased,with statistical significance?p<0.01?;the erythrocyte aggregation index in the group B decreased,with no statistical significance?p>0.05?;compared with group B,the erythrocyte aggregation index in group D decreased,with no statistical significance?p>0.05?;Compared with group A,the erythrocyte aggregation index of group D decreased,the difference was not statistically significant?p>0.05?.Hematocrit:com Pared with group A,the hematocrit in group B decreased,with statistical significance?p<0.01?;the hematocrit in group D decreased,with statistical significance?p<0.05?;the hematocrit in group C increased,with no statistical significance?p>0.05?;compared with group B,the hematocrit in group D decreased,with statistical significance?p<0.01?;Compared with group C,the hematocrit in group D decreased,and the difference was not statistically significant?p>0.05?.3. Uterine Microvascular DiameterCompared with group A,the microvascular diameter in group B contracted significantly,with a statistical difference?p<0.01?;the uterine microvascular diameter in group D contracted,with a statistical difference?p<0.05?;the uterine microvascular diameter in group C expanded,with no statistical difference?p>0.05?;compared with group B,the uterine microvascular diameter in group D contracted,with a statistical difference?p<0.01?.Compared with group C,the diameter of uterine microvasculature in group D was significantly expanded?p<0.01?.4. PGF_2?,PGE₂ and PGF_2?/PGE₂ in UterusPGF_2?:compared with group A,the content of PGF_2?in the uterus of group C increased,with statistical significance?p<0.05?;the content of PGF_2?in the uterus of group B increased,with no statistical significance?p>0.05?;the content of PGF_2?in the uterus of group D increased,with no statistical significance?p>0.05?;compared with group B,the content of PGF_2?in the uterus of group D decreased Compared with group C,the content of PGF_2?in the uterus of group D decreased,and the difference was not statistically significant?p>0.05?PGE₂:com Pared with group A,the content of PGE₂ in the uterus of group B was significantly higher?p<0.01?;the content of PGE₂ in the uterus of group C was significantly higher?p<0.01?;the content of PGE₂ in the uterus of group D was higher?p>0.05?;compared with group B,the content of PGE₂ in the uterus of group D was higher?p>0.05?Compared with group C,the content of PGE₂ in the uterus of group D decreased,but the difference was not statistically significant?p>0.05?.PGF_2?/PGE₂:compared with group A,the value of PGF_2?/PGE₂ in the uterus of group B decreased with statistical significance?p<0.01?;the value of PGF_2?/PGE₂in the uterus of group C decreased without statistical significance?p>0.05?;the value of PGF_2?/PGE₂ in the uterus of group D decreased with no statistical significance?p>0.05?;Compared with group B,the PGF_2?/PGE₂ value of uterus in group D increased,the difference was not statistically significant?p>0.05?;compared with group C,the PGF_2?/PGE₂value of uterus in group D increased,the difference was not statistically significant?p>0.05?.Conclusion:1.Compared with healthy rats,PD rats have the changes of microcirculation,such as the decrease of hemorheology index,the contraction of uterine microvascular diameter,and the increase of PGE₂ and PGF_2?in uterine tissue,which may be thecharacteristics of PD.2.Mild moxibustion can treat PD by improving the microcirculation disturbance of uterus and reducing the content of PGE₂ and PGF_2?in uterus,which shows that it has obvious dilation effect on the contraction of uterine microvascular diameter of PD rats;it has an increasing trend on the decrease of hemorheology index of PD rats;it has a decreasing trend on the increase of PGE₂ and PGF_2?in uterus of PD rats.3.There is a positive correlation between the disturbance of PD uterine microcirculation and the imbalance of PGF_2?/PGE₂ homeostasis,while there is a correlation between the improvement of PD uterine microcirculation by mild moxibustion and the regulation of PGF_2?/PGE₂ homeostasis...