| Objective:The purpose of this study was to analyze the difference of 13 SNPs polymorphism in androgenic alopecia of Mongolian and Han nationality in Inner Mongolia,and to explore the correlation between 13 SNPs polymorphism and androgenic alopecia.Methods:In this study,the candidate gene case-control research strategy was adopted,and androgen alopecia questionnaire and normal control questionnaire were designed uniformly.In the form of questionnaire and blood sampling,50 cases of case group and healthy control group,50 cases of Han case group and 50 cases of control group,39 cases of Mongolian case group and 44 cases of control group were collected.The experimental method is imldrtm multiple SNP typing method,multiple SNP typing kit to extract the whole blood genomic DNA of all samples,establish 8-plex SNPs complex amplification system,and use the technology of snapshot to detect the 13 SNPs loci in the sample genotyping,Plink 1.0.7 software,cgen3.8 software,Gene Mapper 4.1 software,haploview software Software package and other relevant data statistical analysis of all samples of each site HWE value,allele frequency,genotype association analysis,linkage imbalance analysis,haplotype analysis.Results: 1.Unit point analysis.The results of Mongolian case group and control group showed that the correlation analysis of rs17350355 on HDAC9 and rs201571,rs1160312,rs6113491 on linc01432 had statistical difference(P < 0.05),and the analysis of other loci had no statistical difference.In the Mongolian population,the risk of androgenic alopecia was reduced in the individuals with GA genotype in the co dominant model and the individuals with aa-ga genotype in the co dominant model(or = 0.364,95% CI = 0.140-0.948,P = 0.0386;or = 0.398,95% CI 0.162-0.982 in the co dominant model),P = 0.046);the risk of androgenic alopecia was increased in the individuals with TT genotype in the additive model of rs201571(or = 2.195% CI= 1.029-4.287,P = 0.0416),and the risk of androgenic alopecia was increased in the individuals with AA genotype in the additive model of rs1160312(or = 2.244,95% CI= 1.086-4.636,P = 0.029).The results of the Han case group and the control group showed that there was no statistical significance in the frequency of 13 SNPs alleles.2.Chain imbalance analysis.In Mongolian research samples,there are strong linkage in rs3852255,rs13230142,rs12056282,rs2249817,rs17350355 and rs10247184 on HDAC9 gene,rs6137444,rs2180439,rs1998076 on linc01432 gene,rs201571,rs1161032 and rs6113491.Six loci of rs3852255,rs13230142,rs12056282,rs756853,rs2249817 and rs10247184 were closely linked in HDAC9 gene,and five loci of rs2180439,rs1998076,rs201571,rs1160312 and rs6113491 were strongly linked in linc01432 gene.3.Haplotype analysis.In Mongolian samples,the results showed that the haplotype TAA composed of rs201571,rs1160312 and rs6113491 at three loci of linc01432 gene was associated with the increased risk of androgenic alopecia(or =2.247,95% Cl = 1.081-4.671,P = 0.03),while the frequency of other haplotypes was not statistically significant.In the Han population,the frequency of all haplotypes was not statistically significant.Conclusion: 1.Rs17350355,rs201571,rs1160312,rs6113491 and Mongolian in Inner Mongolia are associated with androgenic alopecia.Rs201571,rs1160312 and rs6113491 polymorphism may increase the risk of androgenic alopecia,rs17350355 polymorphism may reduce the risk of androgenic alopecia.2.Individuals carrying haplotype TAA of rs201571-rs1160312-rs6113491 may increase the risk of androgenic alopecia in Inner Mongolia.3.There was no correlation between the polymorphism of 13 SPNs and androgen alopecia in the Han population of Inner Mongolia. |
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