| Objectives FromTreat the T2 DM rats with Shi Jinmo herbal-pair and observe their effects on blood glucose,area under the curve(AUC),C-reactive protein,free fatty acid(NEFA),malondialdehyde(MDA),The effects of superoxide dismutase(SOD)and PI3Kp85?,GLUT4 protein and glycogen content in skeletal muscle tissues,and explore the mechanism of action on insulin resistance of skeletal muscle in T2 DM rats.Methods Fifty SPF-level experiments were conducted with Wistar rats(males)and fed with normal feed+pure water for one week to adapt them to the new living environment.Randomly select 6 animals to be labeled as normal group(Normal),divide them into cages separately,and continue feeding as before.The remaining 44 were fed with high-fat diet+5% sugar water.After 4 weeks,44 rats were intraperitoneally injected with streptozotocin(STZ)at a dose of 35 mg/kg to induce a T2 MD rat model.Fasting blood glucose(FBG)?11.1 mmol/L,postprandial blood glucose(PBG)?16.7 mmol/L is the model standard,excluding death and non-compliance,a total of 30 rats were modeled.According to the random number table method,the 30 model rats were divided into 6 metformin group(Metformin)group,6 T2 DM rat model group(Model)group,and Shijinmo herbal-pair formula groups of low,medium,and high dose(SJML,SJMM and SJMH),with 6 rats in each group.Followed by oral administration of metformin suspension(0.1g/kg),and Shi Jinmo High-dose concentrated drug solution(23.2g/kg original dose),Shijinmo medium-dose concentrated solution(11.6g/kg original dose),Shijinmo low-dose concentrated solution(5.8g/kg native drug amount).Six rats in the normal group without STZ injection were gavaged with normal saline as blank control group.Gavage once a day at 8:00 am,weigh and record every week for 8 weeks,and perform oral glucose tolerance test(OGTT)at 0,4,and 8 weeks of administration,and calculate the area under the glucose curve(AUC),dynamic Observe the state of the rat.At the end of the experiment,blood was collected from the abdominal aorta,centrifuged at 3000r/min for 15 min,and the supernatant was taken.The skeletal muscle tissue of the hind leg was collected and frozen at-80 ?.Serum C-peptide(C-P)and C-reactive protein(CRP)were measured by ELISA,and the insulin resistance index(HOMA-IR)was calculated.Refer to the kit to determine serum free fatty acids(NEFA),malondialdehyde(MDA),superoxide dismutase(SOD)and skeletal muscle glycogen.Western Blot was used to detect PI3Kp85? and GLUT4 protein expression in skeletal muscle.Results 1 After 8 weeks of drug intervention,the rats in the normal group were mentally active,showing an increase in body weight and white and smooth hair;the rats in the model group slowed down,their weight decreased,and their hair color was yellow and hair loss;compared with the model group,the metformin group,SJMH,The weight loss of SHMM and SJML rats was slow,especially in the SJML and metformin groups(P<0.05).2 Effect on FBG,PG2 h and AUC: At the end of the experiment,compared with the model group,the FBG,PG2 h and AUC of the SJMM,SJML and metformin groups were significantly reduced(P<0.05),and the PG2 h and AUC of SJMH decreased(P<0.05)However,the improvement of FBG is not obvious(P>0.05),and the effect of SJML on improving AUC is not different from that of metformin group(P>0.05).3 Effects on C-P and HOMA-IR: After 8 weeks of administration,C-P in model group T2 DM rats decreased and HOMA-IR increased(P<0.05)compared with normal group;T2DM rats ratio in model group,SJMH,SJMM,SJML and Metformin group C-P increased(P<0.05),HOMA-IR decreased,of which SJML and Metformin group was more significant(P<0.05).4 Effects on NEFA,oxidative stress indicators and CRP: specimens were collected 8 weeks after administration,compared with the normal group: NEFA,MDA,CRP of T2 DM rats in the model group increased(P<0.05),and SOD decreased(P<0.05);SOD in SJMH,SJMM,SJML,and metformin groups was no different from the normal group(P>0.05),but MDA,NEFA,and CRP increased(P<0.05).Compared with the model group: NEFA and CRP in SJMM,SJML and metformin groups were significantly decreased(P<0.05),and the effect of SJMH,SJMM and SJML in reducing CRP was not different from that in metformin group(P>0.05);SJMH,SJMM,SJML and metformin The SOD of the group increased significantly(P<0.05)and MDA decreased significantly(P<0.05).5 Effects on PI3Kp85?,GLUT4 protein and glycogen of skeletal muscle: Compared with the normal group,the content of glycogen in muscle of 30 T2 DM rats injected with STZ decreased(P<0.05),the expression of PI3Kp85? and GLUT4 in model group,SJMH was down-regulated(P<0.05),and the level of PI3Kp85? in SJML also decreased(P<0.05).Compared with the model group,PI3Kp85?,GLUT4 protein expression and myoglycogen content of metformin group,SJMH,SJMM and SJML was up-regulated(P<0.05).The expression levels of GLUT4 in SJMM,SJML and metformin groups were not different(P>0.05),and the levels of PI3Kp85? protein in SJMM and metformin groups were not different(P>0.05).Conclusions 1 Shi Jinmo herbal-pair formula can delay the weight loss of T2 DM rats,reduce blood glucose levels,reduce islet function damage in T2 DM rats,improve glucose tolerance and IR.2 Shi Jinmo herbal-pair formula can reduce free fatty acid levels,reduce oxidative stress and inflammatory damage,increase the content of glycongen in skeletal muscle and improve the IR of skeletal muscle in T2 DM rats.3 The mechanism of Shi Jinmo herbal-pair formula for improving the skeletal muscle IR of T2 DM rats may be achieved by up-regulating the levels of PI3Kp85? and GLUT4 protein in skeletal muscle tissue.Figure 6;Table 3;Reference 138... |
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