Study On The Anti-aging Effect And Mechanism Of Hydroxysafflor Yellow A

Aging is an important research field in life sciences.As global aging aggaravates,research progress on delaying aging strategies is urgently required.In recent years,natural medicinal plant derived active ingredients have shown enormous prospects for the development of industrial applications in the field of anti-aging.Hydroxysafflor yellow A(HSYA)is the main active ingredient of Chinese medicine safflower.Several studies have suggested that HSYA can alleviate cardiovascular diseases,neurodegenerative diseases,liver and lung fibrosis diseases and HSYA can inhibit skin photoaging caused by ultraviolet rays.However,the inhibitory effect and molecular mechanism of HSYA on individual aging remains to be explored.In this study,the D-gal-induced subacute aging mouse model was established,and after the intervention of HSYA administration to the mouse model,the weight,growth rate of the mouse and the immune organ index,as well as the antioxidant enzyme activities in serum and liver was simultaniously determined.We also observed the pathological changes of liver tissues of mice to explore the effect of HSYA in delaying the aging process of mice.With the detectiong of changes in the expression level of aging-related genes and proteins in the mouse liver tissue,the molecular mechanism of HSYA was further explored underlining novel anti-aging mechanism which highlights new ideas for the development of new anti-aging and related disease drugs.The specific research results are as follows:1.In this study,using multiple statistical analysis of the mouse body weight,growth rate,thymus and spleen index were determined.Comparing with results of the aging model group,the body weight growth rate,thymus and spleen index of mice in the HSYA treatment group were significantly improved(p <0.05).This shows that HSYA can improve the decrease of immune function and the decrease of body weight growth rate induced by D-gal in mice.2.The activity analysis of antioxidant enzymes SOD,CAT,GSH-PX in mouse serum and liver showed that the serum and liver SOD,CAT,GSH-PX activity in the HSYA treatment group was significantly increased(p <0.05),while the MDA content in the liver was significantly reduced(p <0.05)compared with the aging model group.Theses findings indicated that HSYA can effeciently improve the oxidative stress damage caused by D-gal induced serum and liver in mice.3.During,HE staining and β-galactosidase staining in the mouse liver tissue,the results revealed that the senescence of liver cells in mice due to D-gal induction after HSYA treatment is significantly improved;however,β-Galactosidase staining results showed that the activity of the mouse hepatocyte senescence marker(SA-β-Gal)was significantly reduced after HSYA treatment compared with the mice model aging group,indicating that HSYA can protect liver cells and reduce the D-gal induction by hepatocyte aging.4.Real-time PCR and Western blot quantitative analysis of aging-related genes and proteins in mouse liver tissues suggested that the expression of p16 gene and p16 protein in mouse liver tissues increased significantly after HSYA treatment compared with the aging group.Further validation found that HSYA can regulate p16-Rb signaling pathway-related proteins after treatment,indicating that HSYA can delay the D-gal-induced mouse aging by regulating the p16 gene to inhibit the p16-p Rb pathway.