The Expression And Mechanism Of MicroRNA-3178 In Pancreatic Cancer

Part ? The effect of miRNA3178 on the biological function in pancreatic cancer cellsObjective: This study aims to investigate the expression of mir-3178 in pancreatic cancer cells and normal pancreatic cells,and to explore the effect of mir-3178 on the proliferation,apoptosis and migration of pancreatic cancer cells in vitro.Methods:1.The expression of mir-3178 mRNA in pancreatic cancer cells and normal pancreatic cells were detected by real-time PCR.2.The proliferation of transfected cells was detected by cck-8 assay.The apoptosis and cell cycle of the transfected cells was demonstrated by Flow Cytometry assay.The migration and invasion were evaluated by the Transwell assay.Results:1.Compared with normal HPDE6C7 cell line,the expression of mir-3178 was significantly increased in the AsPC-1 cell line and slightly decreased in the PANC-1 cell line.2.After mir-3178 mimics were transfected into pancreatic cancer AsPC-1 cells,the proliferation and migration of cells were significantly inhibited,and the G0/G1 phase was produced,and the cell apoptosis was induced.3.After mir-3178 inhibitor was transfected into pancreatic cancer AsPC-1 cells,the proliferation and migration of cells are significantly increased,and the apoptosis was inhibited,and the cell cycle was accelerated.Part ? The effect of miRNA3178 on angiogenic factors VEGF,bFGF and endostatin.Objective: To study the relationship between the expression of mir-3178 and the angiogenic factors VEGF,bFGF and endostatin.Methods: Detected the relationship between mir-3178 expression and tumor neovascular endothelial factors by q RT-PCR and Western blot.Results: The overexpression of mir-3178 inhibited the expression of VEGF and bFGF in AsPC-1 cells and enhanced the expression of endostatin.After the mir-3178 inhibitor was transfected into AsPC-1 cells,the expression level of VEGF and bFGF was up-regulated and the expression of endostatin was inhibited.Conclusion:1.The level of mir-3178 expression in pancreatic cancer cell line ASp C-1 is significantly higher than that in normal pancreatic cells.2.Mir-3178 can obviously inhibit the proliferation and migration of pancreatic cancer cells and promote the apoptosis of AsPC-1 cells.3.Mir-3178 can inhibit the expression of VEGF and bFGF,and promote the expression of endostatin,and inhibit the endothelial growth of tumor angiogenesis.In summary,mir-3178 may be a potential target for the treatment of pancreatic cancer.