Effect Of Bushen Huoxue Decoction On Bone Metastases And VEGF, BFGF And MVD In Breast Cancer Of Nude Mice

Objective:Through the left ventricle into nude mice injected human breast cancer MDA-MB-231cells,breast cancer bone metastasis model,build nude mice to observe and explore kidney huoxue soup for breast cancer bone metastases new mechanism of action of angiogenesis.Methods:56 female nude mice,according to the building before weighing the weight of layered,according to a completely random distribution method is divided into eight categories,specific for kidney huoxue soup high concentration,concentration of kidney invigorate the soup group,kidney huoxue soup low concentration group,the azole phosphonic acid group and kidney,activating blood group,the blank group,model group.The blank no intervention group of nude mice,continue as usual,the remaining7 groups for breast cancer bone metastasis model,building method for concentration of left ventricle injection into nude mice is about 1*10~7/ml of human breast cancer MDA-MB-231cells 0.2ml,built in nude mice model of bone metastases.In nude mice after building the strict observation of the specific weight changes and record.After building the next day,except the blank group of nude mice,and the remaining groups of nude mice to drug intervention,intervention methods are as follows:kidney huoxue soup high,medium and low concentration group of nude mice were given crude drug concentration of 40g/Kg,20g/Kg,10g/Kg of kidney invigorate the lavage daily soup;Azole phosphonic acid group of nude mice to 100ug/Kg dose,intraperitoneal injection around the abdomen crossed injections,once a week.Kidney group of nude mice to crude drug concentration of 2g/Kg of kidney in traditional Chinese medicine(kidney invigorate the circulation of soup down party)daily to fill the stomach;Blood group of nude mice to crude drug concentration 0.4/Kg of invigorate the circulation of Chinese traditional medicine(kidney invigorate the circulation of soup down party)daily to fill the stomach;Nude mouse model group to be 0.2ml of normal saline lavage daily.Counted starting from the building,40 days after bone metastasis in breast cancer of each group of nude mice with micro CT test,and record each group of nude mouse breast cancer bone metastases.Micro CT after monitoring,to abdominal aorta in pursuance of nude mice,and executed after the blood will be nude mice.Lesions of groups of nude mice bone tissue on bench,pathological diagnosis,at the same time using HE staining observation of each group of nude mice bone tissue damage,using enzyme-linked immunosorbent(Elisa)method is used to measure each nude mice bone metastases)vascular endothelial growth factor(vascular endothelial growth factor,VEGF)and basic fibroblast growth factor,basic fibroblast growth factor,bFGF),Under a microscope and MVD value measurement.Results:1?After the examination,after building model group at least 100%of all breast cancer bone metastases,bone metastasis rate of the rest of the group nude mice have different degrees of lower;2?Through the left ventricle into nude mice injected human breast cancer MDA-MB-231cells could be successfully built nude mouse breast cancer bone metastasis model;3?Measured in weight changes before and after medication groups of nude mice.By a result the model group had a significantly lower than the rest of the groups of nude mice,nude mice weight contrast difference between groups with statistical significance(P<0.05).4?Model group,nude mice bone metastasesVEGF,bFGFand MVD value is significantly higher than the rest of the group,the difference between group compared with statistical significance(P<0.05);Kidney invigorate the soup the value of high levels of MVD and VEGF,bFGF group of nude mice with azole nude mice significantly reduced to phosphonic acid group,compare the difference between the groups was statistically significant(P<0.05);Kidney huoxue soup high concentration group of nude mice bone metastases)VEGF,bFGF and MVD value compared to the rest of the group decreased significantly,the difference between group compared with statistical significance(P<0.05).Conclusion:1?Through the left ventricle into nude mice injected human breast cancer MDA-MB-231cells could be successfully built nude mouse breast cancer bone metastasis model;1?Kidney huoxue decoction can inhibit the expression of VEGF and bFGF to suppress angiogenesis generated;2?Kidney huoxue decoction can decrease the nude mouse breast cancer bone metastases MVD values of the organization.