| Metastasis of malignant tumors is the main cause of high mortality in cancer patients.Circulating tumor cells(CTCs)play an important role in the metastasis of malignant tumors.Circulating tumor cells are also seen as "seeds" shed from the original tumor.Both in clinically and in experimental animal models,the number of circulating tumor cells increases significantly when malignant tumors begin to metastasize.Circulating tumor cells in the blood need to overcome many obstacles in the process of tumor metastasis to distant organs.Only a few of circulating tumor cells will successfully infiltrate into the distal organ and form metastases.At present,the metastasis ability and mechanism of circulating tumor cells are not fully studied.This project developed a new linear scanning mode based on confocal imaging system to achieve real-time monitoring of circulating tumor cells in vivo.Through theoretical feasibility verification and animal model experiments,it has been proved that the new linear scanning mode of confocal imaging system is a simple,reliable and highly sensitive tool for detecting circulating tumor cells.The effect of blood flow velocity on tumor metastasis was further studied by using the linear scanning model.In terms of stimulation,this study used drug stimulation to improve the calcium level of tumor cells to study its relationship with the metastasis ability of tumor cells.Subsequently,according to the results of drug stimulation,the feasibility of laser in the regulation of tumor cell metastasis ability was further explored.Compared with previous methods of detecting circulating tumor cells,the novel linear scanning mode of confocal imaging system combined with laser to monitor and regulate the metastasis characteristics of circulating tumor cells in real time can achieve controllable,accurate and non-invasive regulation.At the same time,this study also provides a new reference for the mechanism of metastasis of circulating tumor cells. |
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