Effects Of Applied Electric Fields On Microglial Cell Behavior In Neuroprotection And Mechanism

Objective: Traumatic brain injury is a common disease in neurosurgery,and its fatality rate and disability rate rank first in all kinds of trauma.To study the activation process of microglia and the key regulatory mechanism of directional migration,to minimize its damage,and to fully exert its protective function,it is of great significance for central nervous system protection,regeneration and functional recovery after injury.Cell migration and functional changes under the electric field have great potential for nerve repair.This paper mainly discusses the effects of exogenous electric field on the migration and functional status of microglia in vitro and its related mechanisms.Methods: BV-2 microglia were selected for in vitro culture,and the effects of microglia on the movement and inflammatory function of microglia were observed by establishing a DC electric field.Finally,the possible mechanism of action of electric fields on microglia is explored.Results: Microglia showed random motion under the action of electric field.Under the action of electric field,the cells migrated toward the negative electrode,and their migration velocity and tropism were positively correlated with electric field strength.At the same time,actin re-distributes and accumulates on the migration side,and activation of ERK is involved in the electrotaxis movement of microglia.Electric field does not affect the microglial activation of cytokine.Conclusion: Exogenous DC electric field can induce the directional migration of microglia,and it is possible to control the migration of microglia through related mechanisms,and does not affect the change of functional status of microglia,which is the damage of DC electric field in nervous system.The application of repairing the field of regeneration provides a certain scientific basis.