| Angiogenesis that the formation of new blood vessels involved in manyphysiological and pathological processes. In the early1970s, Folkman first proposedtumor growth and metastasis are dependent on angiogenesis, thereby inhibitingangiogenesis may be an effective way to anti-tumor growth and metastasis. Electricfield as an important signal, has been shown to induce endothelial cell directionalmigration, proliferation and re-alignment, which is of great significance to built onthe blood vessels and the extending direction of the blood vessels, and cells can senseelectrical signals preferentially in this process. So if we can change blood vesselgrowth, extends to block the blood vessels to provide nutrients and oxygen to thetumor, and ultimately inhibit tumor growth and metastasis, which will provides abasis and reference new perspective to drug free therapy for cancer.To establish an experiment model, we choose murine aorta ring as the researchobject, analysis of different culture conditions on murine aorta ring angiogenesiseffects, to determine the most suitable conditions cultured murine aorta ring, and thenestablish a murine model of aorta rings. And to study the effect of exogenous electricfields stimulation on characteristics of cell migration and microvessel-like structure inmurine model of aorta rings. The results were as follows:①Successfully established murine aorta ring modelWe culture freshly isolated murine aortic rings by using EGM, RPMI-1640,DMEM and MCDB-131four kinds of media, the growth of microvessel-like structurewas observed and compared in murine aorta rings. The results showed that: murineaorta rings which cultured in EGM medium start to sprouting on the third day, cellsextended to the surrounding radially from the surface of vascular ring; multiple cellend to end to make budding elongation or a branch,which began to appearmicrovessel-like structures, and aortic rings around several buds woven into a meshstructures, then began to regress. And the murine aortic rings which cultured inRPMI-1640, DMEM and MCDB-131three kinds of media start to sprouting in the 4th day, and most of which are scattered cells or short dendritic structures, is moredifficult to focus on taking pictures. Based on the research needs to choose EGMmedium, a murine model of aortic rings was established.②E xogenous micro-DC electric stimulation guidance directed migration of vascularcellsCells were applied to0,200,300mV/mm electric field, respectively. Then, thecharacteristics of cell migration were analyzed. The results showed that in the electricfield, cells migrate towards the anode, and increased galvanotaxis with voltage.Mainly as follows: under0mV/mm,200mV/mm,300mV/mm electrical fieldstimulation，cell displacement speed are0.746±0.034μm/min、1.213±0.036μm/min、1.355±0.034μm/min; Directedness are0.06±0.06、0.628±0.036、0.708±0.029;Persistency are0.517±0.021、0.726±0.012、0.772±0.01, respectively.③Exogenous micro-DC electric stimulation guide the growth of microvessel-likestructures200mV/mm was applied to microvessel-like structures, and then analyze theturning angle and telescopic length of microvessel-like structures. The results showedthat exogenous micro-DC electric stimulation can cause microvessel-like structuredeflected toward the anode. Under200mV/mm electrical field stimulation,microvascular towards the anode, the net turning angle is6.736±2.264°after2h;8.86±2.745°after4h;7.83±2.975°after6h. In addition, we also analyzed the effectsof exogenous micro-DC electric stimulation on the length of microvessel-likestructures, we found that there is no significant difference in effects onmicrovessel-like structures length. |
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