Experimental Study Of Repairing The Sciatic Nerve Defect By Nerve Elongation On Rats

Objective:To investigate the effect of nerve elongation of rats'sciatic nerve on pain release,its possible mechanism and postoperative functional recovery.Methods:?1?36 male Wistar rats were randomly divided into three groups?nerve elongation group,nerve ligation group and nerve non-elongation group?.Autotomy score was dynamically monitored at 3,7,10 and 14 days postoperatively.TNF-?and c-Fos staining were performed at three groups to assess the change of pain during nerve elongation.?2?12 male Wistar rats were randomly divided into two groups?nerve elongation group and nerve grafting group?.Sciatic functional index was dynamically monitored at 3d,7d,10d,2w,3w,4w,5w and 6w postoperatively.The examination of nerve conduction velocity and histological staining were performed at 6w postoperatively.Results:There were significant differences of autotomy score between nerve elongation group and ligation group on 3,7,10 and 14 days postoperatively and significant differences between nerve elongation group and non-elongation group on 10,14 days postoperatively?P<0.05?.The weakest expression of TNF-?were found in spinal dorsal root ganglia?DRG?in nerve elongation group after immunohistochemically staining.No obvious expression of c-Fos was detected in L₄-S₁ in nerve elongation group.There were significant differences of sciatic functional index and nerve conduction velocity between nerve elongation group and nerve grafting group?P<0.05?.The histology detection showed that newborn myelin sheaths were found in nerve elongation group.Conclusion:Nerve elongation does not cause obvious pain,conversely,nerve elongation can avoid pain.Nerve elongation group obtains better functional recovery than nerve grafting group.Objective: To investigate the effect of nerve elongation of rats' sciatic nerve on pain release and its possible mechanism.Methods: 36 male Wistar rats were randomly divided into three groups?nerve elongation group,nerve ligation group and nerve non-elongation group?.Autotomy score was dynamically monitored at 3,7,10 and 14 days postoperatively.TNF-? and c-Fos staining were performed at three groups to assess the change of pain during nerve elongation.Results: There were significant differences of autotomy score between nerve elongation group and ligation group on 3,7,10 and 14 days postoperatively and significant differences between nerve elongation group and non-elongation group on 10,14 days postoperatively?P<0.05?.The weakest expression of TNF-? were found in spinal dorsal root ganglia?DRG?in nerve elongation group after immunohistochemically staining.No obvious expression of c-Fos was detected in L4-S1 in nerve elongation group.Conclusion: Nerve elongation does not cause obvious pain,conversely,nerve elongation can avoid pain.Objective: To investigate postoperative functional recovery after nerve elongation.Methods: 12 male Wistar rats were randomly divided into two groups?nerve elongation group and nerve grafting group?.Sciatic functional index was dynamically monitored at 3d,7d,10 d,2w,3w,4w,5w and 6w postoperatively.The examination of nerve conduction velocity and histological staining were performed at 6w postoperatively.Results: There were significant differences of sciatic functional index and nerve conduction velocity between nerve elongation group and nerve grafting group?P<0.05?.The histology detection showed that newborn myelin sheaths were found in nerve elongation group.Conclusion: Nerve elongation group obtains better functional recovery than nerve grafting group.