Correlation Between Hbx Mutation And Serum HGF In Patients With Hbv-related Hepatocellular Carcinoma

Objective: To explore the correlation between the mutation of Hepatitis B virus X gene(HBX)in patients with HBV-related hepatocellular carcinoma and the expression of hepatocyte growth factor(HGF)in the serum of the infected patients.Methods: The 155 serum samples of patients with HBV infection and HBV-DNA quantification ?10 ^ 3 copies / ml were collected in the Second Affiliated Hospital of Chongqing Medical University from April 1st,2018 to October 31 st,2018(including 55 patients in hepatitis group,50 patients in cirrhosis group,50 patients in hepatocellular carcinoma group).HBV-DNA was extracted from the patient's serum,and the HBX gene was amplified using the nested PCR method,and the product was sequenced.The sequencing results were compared with the wild-type HBX gene by seqman software to analyze the mutation site.Enzyme-linked immunosorbent assay was used to detect the concentration of HGF in the serum of patients.The data was processed by t-test,chi-square test,rank sum test,Kaplan-Meier method,log-rank test,receiver operatingcharacteristic curve,Cox proportional hazards regression model and other methods.Results: Analysis of the common HBX mutation sites in the serum of155 patients were: G1613 A,G1635A,A1652 G,C1653T,C1673 T,G1676A,C1730 G,T1753G,A1762 T / G1764 A,and the detection rate of G1635 A was significantly different among the three groups(P<0.05).The serum HGF concentration in the hepatocellular carcinoma group was significantly higher than that in the other two groups,and had a significant statistical significance(P<0.05).Patients with G1635 A,A1652G,C1673 T,and C1730 G mutations in the hepatocellular carcinoma group had lower serum HGF levels than those without detection(P values were 0.000,0.000,0.001,0.002 respectively,and P<0.05 were statistically significant),while patients with T1753 G and A1762 T / G1764 A mutations had higher levels of serum HGF than those without detection(P values were 0.002,0.015 respectively,and P <0.05 were statistically significant).A total of 48 patients died during the 1-year follow-up,and the cumulative survival rate of the patients differed among the groups and had a significant statistical significance(P<0.05).Serum HGF levels had a significant effect on the prognosis of patients(HR 3.967;95%CI: 2.061-7.637;P=0.000),the higher the serum HGF concentration,the lower the survival rate of patients.The detection rate of G1635 A mutation in the dead patients was significantly different among the groups(P <0.05).Conclusion: The high level of serum HGF concentration may be related to the occurrence and development of liver cirrhosis and hepatocellular carcinoma,as well as to a lower survival rate.G1635 A,A1652G,C1673 T,and C1730 G mutations may be associated with decreased serum HGF concentration,while T1753 G and A1762 T / G1764 A mutations may be associated with increased serum HGF concentration.G1635 A may have a potential link with reducing the risk of liver cancer.