| Objective1.Preparation of a stable and reliable diabetic foot rat model;2.To explore the relationship between diabetic foot disease and the level of serum platelet-derived growth factor,high mobility group box 1 in rats.Methods1.50 male SD rats of 120 ~ 140 g were selected and 40 of them were randomly divided into 2 groups: experimental group(n=20)and control group(n=20),and 10 normal SD rats were selected as the blank control group(n=10).In the experimental group,STZ 35mg/kg was intraperitoneally injected after 2 months of high-sugar and high-fat feed.In the control group,STZ 35mg/kg was intraperitoneally injected after 2months of normal feed.In the blank control group,sodium citrate buffer was intraperitoneally injected after 2 months of normal feed.Blood glucose of rats was monitored on time and regularly for 2 weeks.Rats in each group were scalded by hot plate apparatus,and were inoculated with Staphylococcus aureus suspension to observe the progress and outcome of ulcer.2.70 SD rats were randomly divided into experimental group(n = 30)and control group(n = 30).Diabetic foot rat model was established in experimental group,diabetic foot rat model was established in control group,and 10 normal male SD rats were fed with common feed without special treatment.The levels of serum HMGB1 and PDGF were measured before and after the injection respectively,to explore the correlation between the concentrations of serum HMGB1 and PDGF and diabetic foot disease.All data were statistically analyzed using SPSS 20.0 software.Results1.The success rate of diabetic foot model of SD rats in the experimental group was 70%,and the mortality rate was 10%;2.Compared with the control group,the serum HMGB1 level of rats in the experimental group was significantly higher than that in the control group and the blank group,and the difference was statistically significant(P < 0.05).Compared with the blank group,the serum HMGB1 level of the control group was significantly higher than that of the blank group,and the difference was statistically significant(P< 0.05).3.Compared with the control group,the serum PDGF level of the experimental group was significantly higher than that of the control group and the blank group,and the difference was statistically significant(P < 0.05).Compared with the blank group,the serum HMGB1 level of the control group was significantly higher than that of the blank group,and the difference was statistically significant(P < 0.05).Conclusion1.A stable and reliable experimental animal model of diabetes can be established by feeding SD rats with high sugar and high fat diet for 2 months,injecting STZ35mg/kg intraperitoneally,scalding with hot plate apparatus and inoculating pathogenic bacteria locally.The success rate of diabetic foot rat model is high and the mortality rate is low;2.The level of serum HMGB1 in diabetic rats is higher than that in normal rats,which indicates that HMGB1 has a certain relationship with the onset of diabetes;the level of serum HMGB1 in diabetic foot rats is higher than that in diabetic rats,which indicates that HMGB1 is closely related to the onset of diabetic foot;3.The level of serum PDGF in diabetic rats is higher than that in normal rats,which indicates that PDGF is related to the pathogenesis of diabetes;the level of serum PDGF in diabetic foot rats is higher than that in diabetic rats,which indicates that PDGF is closely related to diabetic foot. |
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