Study On The Hepatoprotective Effects Of Aronia Melanocarpa On Alcoholic Liver Disease

Alcoholic liver disease(ALD)is a liver disease with high morbidity and mortality that is widely distributed worldwide.Aronia melanocarpa(AM)is native to Europe and North America;anthocyanins are important active substances in it.AM presents both high nutritional value and medicinal value.With the increase of alcohol consumption year by year,alcoholic liver disease has become a pressing issue all over the world,which needs to be urgently resolved.In this study,AM was used as the treatment.By establishing a chronic alcohol-induced liver injury model in mice,the protective effect and mechanism of AM in alcohol-induced liver injury were explored In this study,through systematic analysis and evaluation,AM contains high levels of anthocyanin,18 types of fatty acids,17 types of amino acids,eight types of minerals,and three types of nucleotides.Through establishing a mice model of alcoholic liver disease by chronic administration of alcohol for 24 weeks,the protective effect of AM on liver damage induced by alcohol was explored.The results show that AM can effectively alleviate the increase of mouse organ index caused by alcohol;significantly reduce the liver injury marker factors alanine aminotransferase/aspartate aminotransferase(ALT/AST)ratio,triglyceride(TG),acetyl acetyl-CoA synthetase(AACS)and low-density lipoprotein(LDL)levels,while increasing high-density lipoprotein(HDL)levels,reducing the burden of lipid accumulation in mice Vivo.In addition,AM can significantly alleviate fat droplet accumulation in the liver and pathological changes in kidney,spleen,and heart tissues.ELISA results show that after six weeks of intake,AM can effectively regulate the abnormal change of oxidative stress-related factors superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),amyloid P component(APCS),reactive oxygen species(ROS),glutathione peroxidase(GSH-Px),microsomal glutathione S-transferase 3(MGST3),coenzyme 3(CoQ3),cytochrome P4508B1(CYP8B1)and inflammatory response-related factors interleukin-2(IL-2),interleukin-4(IL-4),interleukin-6(IL-6),fibrinogen-like protein 1(FGL1),thioesterase 4(THEM4),fibrinogen y(FGG)caused by chronic alcohol administration for 24 weeks.Western blot analysis showed that AM up-regulated the expression of the antioxidant enzymes CAT,SOD,and heme oxygenase-1(HO-1)by regulating the expression of nuclear factor E2-related factor 2(Nrf2)and Kelch-like ECH-associated protein 1(Keap-1)in order to regulate oxidative stress;In addition,AM can further activate the phosphatidylinositol-3-hydroxykinase/protein kinase B(PI3K/Akt)pathway by up-regulating signal transducer and activator of transcription 3(STAT3),alleviating the inflammatory response;At the same time,the PI3K/Akt pathway can lead to the activation of Nrf2 and thus resist the generation of oxidative stress.This study shows that six weeks of AM administration can alleviate oxidative stress in mice induced by 24-week alcohol administration and relieve liver damage.The mechanism may be related to the PI3K/Akt and Nrf2/HO-1 signaling pathways.