Correlations Between 3.0T MR Features And Molecular Marker Ki67?P504s In Prostate Cancer

Part ? Correlation Between Parameters of Diffusion Weighted Imaging,Dynamic Contra st-enhanced Magnetic Resonance Imaging and the Expression of Ki67 Protein in Prostate CancerObjective:To investigate the correlation between diffusion-weighted imaging?DWI?and dynamic contrast-enhanced magnetic resonance imaging?DCE-MRI?parameters of prostate cancer and Ki67 protein expression.Methods:A total of 93 patients with prostate disease?including prostate cancer ? benign prostatic hyperplasia and/or chronic prostatitis?confirmed by pathology were enrolled in the study.Multi-modality magnetic resonance imaging?mp MRI?was performed to obtain the time-signal intensity?SI-T?curve and peak time?Tmax?of the prostate cancer group and non-prostate cancer group.Maximum enhancement SI?SImax?,fastest enhancement rate?Rmax?and automatically generate apparent diffusion coefficient?ADC?values were obtained;immunohistochemical was used to detect the expression of Ki67 and its correlation with MRI parameters.Result:The ADC values,Tmax,SImax and Rmax of the prostate cancer group and the non-prostate cancer group were?0.76±0.16?x10^-3 mm²/s,?18.61±4.33?s,?1.74±0.39?%,?21.03±14.72?%,respectively;and?1.36±0.19?x10^-3mm²/s,?37.45±19.51?s,?1.32±0.45?%,?10.11±6.22?%,the dif ference between the two groups was statistically significant?P < 0.001?.The ADC values,Tmax,SImax and Rmax of the Ki67 negative group were?1.26±0.25?x10^-3mm²/s,?34.44 ±20.19?s,?1.37±0.47?%,?9.92±8.87?%,respectively;Ki67 low expression group were?0.95±0.36?x10^-3mm²/s,?23.74±12.44?s,?1.62±0.40?%,?19.28±12.41?%;Ki67 high expression group were?0.70±0.06?x10^-3mm²/s,?18.87±4.74?s,?1.85±0.45?%,?23.64±19.51?%.For Ki67 negative group,Ki67 low expression group and Ki67 high expression group,there were significant differences between these groups?H= 32.588,18.719,10.867,18.595;P < 0.05?.However,there was no significant difference between the Ki67 low expression group and the Ki67 high expression group?P > 0.05?.ADC values and Tmax were negatively correlated with Ki67?r =-0.50 and-0.37,P < 0.01?;SImax and Rmax were positively correlated with Ki67?r = 0.29 and 0.36,P < 0.01?.Conclusion:The ADC value of prostate cancer and DCE-MRI perfusion index are correlated with Ki67.The biological characteristics of prostate cancer can be evaluated non-invasively,and it can be used as an imaging biological indicator to predict the degree of tumor malignancy.Part ? Multi-modality MRI parameters correlate with the expression of the P504 s protein in prostate cancerObjective:To evaluate the expressions of P504 s,34?-E12,and P63 protein in PCa and the correlation between the parameters of multi-modality MRI and the expression of P504 s.Methods:We retrospectively analyzed the multi-modality MRI data on 43 PCa and 64 nonPCa patients.We obtained the signal intensity-time?SI-T?curve,maximum SI?SImax%?,time to SImax?Tmax?,rate of maximum enhancement?Rmax?,and automatically generated apparent diffusion coefficient?ADC?values by conventional,diffusion-weighted and dynamic contrast-enhanced MRI,and determined the expressions of P504 s,34?-E12 and P63 in the prostatic tissues of the patients by immunohistochemistry.Result:Statistically significant differences were observed between the PCa and non-PCa groups in the positive expressions of P504s?83.7% vs 0%,P < 0.05?,34?-E12?25.6% vs 91.0%,P< 0.05?and P63?25.6% vs 86.0%,P < 0.05?in the prostatic tissues,the ADC value([0.83±0.22]vs [1.34±0.28]x10^-3mm²/s,P < 0.05),Tmax?[21.30±10.78] vs [50.22±36.31] s,P < 0.05?,SImax?[1.75±0.39]% vs [1.24±0.41]%,P < 0.05?,and Rmax?[20.20±15.50]% vs [7.98±6.25]%,P <0.05?.The expression of P504 s was correlated negatively with the ADC value and Tmax?r =-0.60 and-0.37,P < 0.01?but positively with SImax and Rmax?r = 0.50 and 0.45,P < 0.01?.Conclusion:The parameters of multi-modality MRI are correlated with the expression of P504 s in PCa and can be used as imaging biomarkers for predicting the degrees of its malignancy.