Sulfur Mustard Induced Oxidative Stress Response In Rats With Acute Lung Injury

Objective A rat model of acute lung injury with Sulfur mustard（SM）at equal toxic dose(1LD₅₀)was established,and the rats were exposed to peritoneal cavity and trachea through two different routes.The different expression of oxidative stress related proteins in each experimental group were observed under the two routes and at different time points.Methods A total of 176 male Sprague-Dawley（SD）rats in specific pathogen free（SPF）level were randomized to the abdominal Sulfur Mustard group,the abdominal propylene glycol group,the tracheal sulfur mustard group and the tracheal propylene glycol group,and the normal group was detected by ELISA.The levels of antioxidant enzymes and the changes in four antioxidant enzymes in the alveolar space were measured by the immunohistochemical method.Results In the intraperitoneal SM group,the serum superoxide dismutase,catalase,and glutathion peroxidase levels were increased compared with the tracheal SM group at a period of time（P<0.05）.The positive of CuZn-superoxide dismutase,Mn-superoxide dismutase,paraoxonase-1,and apolipoprotein-1 expression of the alveolar septum in the intraperitoneal SM group were higher than tracheal SM group,the intraperitoneal propylene glycol group,the tracheal propylene glycol group,and normal group（P<0.05）.The four proteins in the peritoneal sulfur mustard group and tracheal mustard gas group increased with time.Conclusion After the rats were exposed to SM with an equal toxic dose,contents and expressions of antioxidant enzymes in serum and alveolar septum-in the two rat models,namely acute pulmonary injury)were different,and were affected by the time effect.This can provide new ideas for of SM-induced acute pulmonary injury on the treatment.