The Protective Effect Of Oral Rehydration Solution ? On Multiple Organ Dysfunction In Exertional Heatstroke Rats

Objective:To investigate the protective effect of Oral Rehydration Solution III?ORS III?on multiple organ dysfunction in exertional heatstroke?EHS?rats.Methods:51 male SD rats were ramdomly divided into 4 groups:Control?n=13?,EHS?Exertional heatstroke??n=13?,EHS+Water?n=12?,EHS+ORS?Oral Rehyration solution??n=13?.All rats received adapative trainning 7 days before the experiment.On the 8^th day,rats from group EHS+Water were injected by gavage with 20ml/kg water.Rats from group EHS+ORS were given the same volume of Oral Rehydration Solution III?ORS III?.The EHS model was built through exercising under hot enviroment.We considered core temperature of 40.5?as the onset of exertional heatstroke.After 6 hours,the concentrations of serum creatine phosphokinase-MB?CK-MB?,lactate dehydrogenase?LDH?,alanine transaminase?ALT?,aspartate transaminase?AST?,Serum creatinine?SCr?,blood urea nitrogen?BUN?,serum potassium?K⁺?,serum sodium?Na⁺?and serum chloride?Cl^-?were detected by Antomatic Chemical Analyzer.Serum neutrophil gelatinase-associated lipocalin?NGAL?was measured using an NGAL ELISA Kit.Serum intestinal fatty acid-binding protein?I-FABP?were measured by I-FABP ELISA Kit.Light microscopy was used for kidney structural analysis.The data were analyzed using SPSS21.0.For data which followed a normal distribution,values were expressed as mean±SD.Skewed data were expressed as median?25%–75%?quartiles.Comparisons for parametric data among groups using a one-way analysis of variance?ANOVA?,following LSD multiple comparisons.For non-parametric data,significance levels were from Kruskal–Wallis ANOVA,A value of P<0.05 was considered statistically significant.Results:The LDH level in group EHS rats was higher than group Control?P=0.032?,the concentrations of ALT,AST,Na⁺and Cl^-in group EHS were significantly higher than group Control?P<0.001?,while the concentration of K⁺in group EHS+ORS was lower than group EHS?P=0.002?.NGAL levels in Group EHS were higher than Group Control?P<0.001?.NGAL concentrations in group EHS+Water were no different from Group EHS?P>0.05?,but NGAL levels were significant between Group EHS and Group EHS+ORS?P=0.012?.For the intestinal barrier function assessment,I-FABP level in group EHS was higher than group Control?P<0.01?,the same conclusion was observed between group EHS and group EHS+Water?P<0.05?,as well as group EHS and EHS+ORS?P<0.01?.Renal histopathologies of rats in Group EHS and Group EHS+Water showed flattened lumens filled with eosinophilic materials.The damage was milder in Group EHS+ORS,in which injured tubules showed degeneration of the tubular epithelium and sloughing of the brush border membrane.Conclusions:1?EHS could lead to liver and intestinal barrier dysfunction,as well as ion imbalance.2?Pre-treatment of ORS III could alleviate the intestinal function and ion disorder caused by EHS in rats.It can lower the serum K⁺to some extent.ORS III could alleviate the kidney injury in EHS rats.However,the ORS III failed to protect heart and liver from EHS.