Evaluation Of The Effectiveness Of Two Methods For Detecting Carious Dentin:an In Vitro Study

Backgrounds:Caries is a common disease affecting oral health.Usually,the way to treat caries is to remove the diseased tissue and then fill it with materials.Taking the most common dentine caries in the clinic as an example,the pathological changes are divided into a transparent layer,a demineralized layer,a bacterial invasion layer and a necrotic disintegrating layer.From the pathological point of view,the bacterial invasion layer and the necrotic disintegrating layer are the infection layers,and the clinical treatment needs to be removed;the transparent layer and the demineralized layer have not been infected to cause damage,and should be retained as much as possible.Uninfected layers that have not been removed or excessively removed from healthy tissue have an impact on treatment outcomes.Therefore,it is very important to accurately detect the boundaries between damaged tooth tissue and healthy tissue.At present,the methods used to study the layered structure of dental caries mainly include optical microscope,scanning electron microscope,laser scanning confocal microscope and three-dimensional shape measurement laser microscope,and each has its own advantages and disadvantages.Considering the three-dimensional shape measurement laser microscope to analyze the whole and local microstructure of dentin caries,it is possible to more clearly observe the four-layer structure according to the color difference.Therefore,this method was selected to compare the effects of two methods for detecting smashed tooth tissue with FACE and caries detector dyes.Objective:The purpose of this study is to investigate the pathological effects of FACE and caries detector dyes as tools for detecting of dental caries by means of a three-dimensional shape measurement laser microscopy system.To determine the caries detection effect(CRE=RC/IC)and minimally invasive potential(MIP=PC/IC)of the two methods,it provides an objective reference for oral clinical decompression.Methods:Collect thirty-two teeth that meet the inclusion criteria of the experiment,and cut each tooth into two samples along the center of the smashing center with a hard tissue cutter.The two samples are randomly divided into two groups: A and B.Then,the three-dimensional shape measurement laser microscopy system was used to scan the layered structure of the two groups before decomposing,and the area of the theoretically damaged tooth tissue(IC)in the two samples was calculated by Auto CAD2019 software.The method of detecting the smashed tooth tissue was used to remove the humus of the dentin.Then,the three-dimensional shape measurement laser microscopy system was used to scan the layered structure of the dehumidified tooth tissue,and Auto CAD2019 software was used to calculate the prepared cavity area(PC)and residual cavity area(RC).Statistical description and statistical analysis were performed using SPSS 20.0,and the description of quantitative data was expressed by ±s.The difference between the means of the two treatment groups was compared using the independent sample t test.The correlation of the variables was expressed by Pearson,s correlation coefficient,and the test level was double ɑ = 0.05.The comprehensive detection effect of two methods for detecting smashed tooth tissue was compared with the FACE and Caries detector dyes.Results:1.Using scatter plots to describe the correlation between the theoretical de-corrosion area(IC)and the residual cavity area(RC),the results show that the two variables have no linear correlation,R2 = 0.010,poor fit,and both of their pearson,s correlation coefficient was 0.101,P=0.426,which was not statistically significant.2.Measure the theoretical decontamination area(IC),the prepared cavity area(PC),and the residual cavity area(RC)between different treatment groups,and calculate the caries detection effect of two methods for assisting the detection of dentin enthalpy(CRE=RC/IC)and minimally invasive potential(MIP=PC/IC),using ± s description,the two sets of data were tested for normality and homogeneity of variance,each group of data met normality and homogeneity of variance(P> 0.05),independent sample t test,the results showed that the prepared cavity area(PC)of the two groups was statistically significant(P = 0.015);the minimally invasive potential(MIP)of the two groups was statistically significant(P < 0.001).3.The box plot is used to represent the distribution of the two groups of minimally invasive potential(MIP)data,and the minimum,first quartile,median,third and fourth and the maximum.It indicates that the median MIP value of the FACE group is closer to1,which has better minimally invasive potential.Conclusion:The detection effect of the group A(FACE)was not significantly different from that of the group B(Caries detector dyes),but the minimally invasiveness of the group A(FACE)was better than that of the group B(Caries detector dyes).Therefore,FACE can be used as a relatively effective tool for assisting the detection of damaged tooth tissue,which is a relatively reliable evaluation method in the process of removing caries.So,it can provide an objective reference for oral clinicians in terms of removal.