Study On The Effect Of Transforming Growth Factor-β1 On Intraplaque Angiogenesis And Stability Of Carotid Atherosclerosis Plaque

Objective:To investigate the effect of competitive inhibition ofβ-catenin/TCF andβ-catenin/FoxO1 signal downstream of transforming growth factor-β1 on angiogenesis and plaque stability in carotid plaque.Methods:1.Plaque tissue of patients with carotid stenosis after CEA（n=21）.Vulnerable and stable plaques were identified by hematoxylin-eosin（HE）,and the expression sites and levels of PECAM1/CD31 and VE-Cadherin in each tissue were analyzed by immunohistochemical（IHC）.2、Model of carotid stenosis was established in ApoE^-/-mice（Group A、B、C、D,n=12/group）.Group A:TGF-β1（50ug/kg）+normal saline（5mg/kg）;Group B:TGF-β1（50ug/kg）+ICG-001（5mg/kg）;Group C:normal saline（50ug/kg）+ICG-001（5mg/kg）);Group D:normal saline（5.05mg/kg）.All group were treated by their own treatment for 2 weeks,then the carotid tissues were taken.After hematoxylin-eosin（HE）,vulnerable and stable plaques were divided into vulnerable and stable plaques according to pathological characteristics.The expression sites and levels of PECAM-1/CD31 and VE-Cadherin in each tissue were stained by immunohistochemical（IHC）.All data were analyzed by Imageplus6.0 and SPASS25.0 statistical software.Results:The content of TGF-βin stable plaque is higher than that in vulnerable plaque.But the expression of VE-Cadherin and CD31 in stable plaque are less than vulnerable plaque（p<0.05）.In animal experiment,the rate of vulnerable plaque in group A（TGF-β）and group B（TGF-β+ICG-001）is lower than that in blank control group.And the positive expression of VE-Cadherin and CD31 in group B（TGF-β+ICG-001）are lower than that in group A（TGF-β）and blank control group（p<0.05）.Conclusion:Specific blockingβ-catenin/TCF downstream of TGF-βcan inversely enhance the inhibitory effect ofβ-catenin/FoxO1 on carotid atherosclerosis intraplaque angiogenesis,thereby having a positive impact on stabilizing plaque.