Study On The Effect Of Transforming Growth Factor-?1 On Intraplaque Angiogenesis And Stability Of Carotid Atherosclerosis Plaque

Objective:To investigate the effect of competitive inhibition of?-catenin/TCF and?-catenin/FoxO1 signal downstream of transforming growth factor-?1 on angiogenesis and plaque stability in carotid plaque.Methods:1.Plaque tissue of patients with carotid stenosis after CEA?n=21?.Vulnerable and stable plaques were identified by hematoxylin-eosin?HE?,and the expression sites and levels of PECAM1/CD31 and VE-Cadherin in each tissue were analyzed by immunohistochemical?IHC?.2?Model of carotid stenosis was established in ApoE^-/-mice?Group A?B?C?D,n=12/group?.Group A:TGF-?1?50ug/kg?+normal saline?5mg/kg?;Group B:TGF-?1?50ug/kg?+ICG-001?5mg/kg?;Group C:normal saline?50ug/kg?+ICG-001?5mg/kg?);Group D:normal saline?5.05mg/kg?.All group were treated by their own treatment for 2 weeks,then the carotid tissues were taken.After hematoxylin-eosin?HE?,vulnerable and stable plaques were divided into vulnerable and stable plaques according to pathological characteristics.The expression sites and levels of PECAM-1/CD31 and VE-Cadherin in each tissue were stained by immunohistochemical?IHC?.All data were analyzed by Imageplus6.0 and SPASS25.0 statistical software.Results:The content of TGF-?in stable plaque is higher than that in vulnerable plaque.But the expression of VE-Cadherin and CD31 in stable plaque are less than vulnerable plaque?p<0.05?.In animal experiment,the rate of vulnerable plaque in group A?TGF-??and group B?TGF-?+ICG-001?is lower than that in blank control group.And the positive expression of VE-Cadherin and CD31 in group B?TGF-?+ICG-001?are lower than that in group A?TGF-??and blank control group?p<0.05?.Conclusion:Specific blocking?-catenin/TCF downstream of TGF-?can inversely enhance the inhibitory effect of?-catenin/FoxO1 on carotid atherosclerosis intraplaque angiogenesis,thereby having a positive impact on stabilizing plaque.