Preliminary Study Of Heat Shock Protein27 Expression And Its Correlation With Stage,Risk And Cisplatin Resistance In Neuroblastoma

Objective:We aimed to investigate the expression of heat shock protein 27(Hsp27)in children with neuroblastomas.Further,we sought to analyze the relationship of Hsp27 expression and stage,risk,cisplatin resistance.Methods:Immunohistochemical(IHC)staining was performed to detect the expression of Hsp27 protein in 90 neuroblastomas(NBs),24ganglioneuroblastomas(GNBs),and 20 ganglioneuromas(GNs).Quantitative real-time polymerase chain reaction PCR(RT-qPCR)examinations were performed to detect the expression of mRNA in 59NBs/GNBs,5 GNs,neuroblastoma cell lines,and drug-resistant cell lines.Immunohistochemical staining,PCR amplification sequencing were used to examine the expression or mutation of drug-related genomic markers in18 NBs/GNBs.Results:The positive expression rates of Hsp27 in NBs/GNBs were significantly higher than that in GNs(64.9%vs.0,χ~2=28.994,P<0.05).In NBs/GNBs,positive Hsp27 expression was associated with tumor staging,risk stratification,metastasis,and prognosis(P<0.05),but was not associated with age,sex,Shimada classification,N-MYC(P>0.05).The RNA levels of Hsp27 in NBs/GNBs were higher than those in GNs(3.763±1.854 vs.2.476±0.698,P>0.05).Five genomic markers of anti-neoplastic agents were examined,and Hsp27 expression was found to be related to platinum resistance.The RNA levels of Hsp27 in the cisplatin-resistant cell line were significantly higher than those in the neuroblastoma cell line(F=16.246,P<0.01).Conclusion:Hsp27 expression was associated with tumor staging,risk stratification,metastasis,and prognosis.Therefore Hsp27 may promote tumorigenesis,progression,and metastasis.Furthermore,Hsp27 expression is related to cisplatin resistance.Further studies with larger sample sizes areneeded to verify these correlations.