The Role And Mechanism Of HSP27 In Drug Resistance Of Cisplatin In Ovarian Cancer Cells

Objective: To investigate the expression and role of HSP27 in cisplatin-sensitive ovarian cancer cell OV2008 and cisplatin- resistant ovarian cancer cell C13K.Methods: to examine the expression of HSP27 gene in ovarian cancer, ovarian cancer comprehend cisplatin sensitive cell line OV2008 cell and cisplatin resistance cell line C13K cell; siRNA and plasmid pEGFP- C1-HSP27 were synthesized, then were transfected into human ovarian cancer cells C13K and OV2008 by lipo2000, mRNA and protein expression of HSP27 at different times after transfection were measured by real-time PCR and western blot, check the drug sensitivity to cisplatin by MTT and flow cytometry.Results: (1) the expression of HSP27 in C13K cells were significantly higher than those in OV2008 cells (p<0.05). (2) Transfection of siRNA 48h, The 50% inhibition concentration ( IC50 ) to cisplatin of C13K transfected with siRNA was(7.7±0.3)μmol/L,obviously lower than those of empty-vector transfected cells and untransfected cells[(17.6±0.3),(17.0±0.2)μmol/L, p<0.05]; Transfection with HSP27 full length plasmid p-HSP27 48h, The 50% inhibition concentration ( IC50 ) to cisplatin of OV2008 with p-HSP27 was(15.2±0.3)μmol/L, obviously higher than those of empty-vector transfected cells and untransfected cells [3.70±0.3),(3.5±0.2)μmol/L, p<0.05] . (3) transfection with siRNA and p-HSP27 48h, and then with cisplatin of different 24h, result display that C13K cell apoptosis rate in transfected with siRNA was significantly increased, than empty vector and non-transfected C13K cells (p <0.05); apoptosis rate of OV2008 in transfected with p-HSP27 was significantly decreased than transfected with empty vector and non-transfected OV2008 cells(p <0.05).Conclusion: HSP27 gene expressions in C13K and OV2008 cells can affect the strength of their sensitivity to cisplatin, suggesting that HSP27 gene may be a potential target for therapy on cisplatin-resistant ovarian cancer.