| The ingredients contained in traditional Chinese medicine are very complicated,and many of the ingredients exhibiting curative effects are not even known,which is difficult for the quality control of traditional Chinese medicine.High performance liquid chromatography?HPLC?has become an important analytical method in the research of traditional Chinese medicine due to its high efficiency,fastness,stability and high sensitivity,and is widely used in quality control of traditional Chinese medicine.This paper intends to use HPLC to study a new method for determining the content of active ingredients in Chinese medicine Codonopsis Radix and Honeysuckle,and it is expected to provide a reference for its quality control.The paper consists of two parts,a research report and a review.The review briefly outlines the techniques commonly used in quality control of traditional Chinese medicine.In the first part of the research report,an HPLC method for simultaneous determination of eight active ingredients in Codonopsis Radix was established,and the influence of mildew on the active ingredients was investigated.The second part,by using pre-column derivatization HPLC to analysis Honeysuckle polysaccharide content and monosaccharide composition in different period Honeysuckle.The first part,simultaneous determination of eight Active Ingredients in Codonopsis pilosula by HPLC.Objective The HPLC method was used to simultaneously determine the contents of eight active ingredients in Codonopsis Radix,including Lobetyolin,niacin,adenosine,syringin,succinic acid,ferulic acid,atractylenolide III and5-hydroxymethylfurfural?5-HMF?.And the effect of mildew on the quality of traditional Chinese medicine was investigated by investigating the content of active ingredients after mildew.Method The HPLC system consisting of Spex Amethyst C18 column?4.6 mm×250 mm,5?m?and a gradient elution system of Methanol?A?-phosphoric acid solution?B?as the mobile phase was adopted.The flow rate was set at 1 mLˇmin-1,The UV detection wavelength was switched,and the column temperature was30?.Results The experimental results show that Mass concentration of8 components had good linear relation with peak areas?R?0.9991?.And average recovery rates were from 97.07%to 103.81%with RSD?2.6%.Conclusion The method is simple,accurate,rapid,and has good reproducibility,which is suitable for simultaneous determination of eight active ingredients in Codonopsis Radix in the experiment.It can provide reference for the quality research of Codonopsis.the second part,by using pre-column derivatization HPLC to analysis contents of Honeysuckle polysaccharides and compositions of monosaccharide in different period.Objective To establish a pre-column derivation High Performance Liquid Chromatography?HPLC?method to determine contents of Honeysuckle polysaccharides and compositions of monosaccharide in different period.of monosaccharide compositions in Honeysuckle polysaccharide,and analyze the monosaccharides differences in different period Honeysuckle.Method The honeysuckle polysaccharide was extracted by Water-extraction and alcohol-precipitation method,and hydrolyzed into monosaccharides with trifluoroacetic acid.The hydrolyzed monosaccharide product was derivatized with 1-phenyl-3methyl-5-pyrazolone?PMP?,and determined by HPLC.Result The honeysuckle polysaccharide is mainly composed of mannose,rhamnose,galacturonic acid,glucose,galactose,xylose,arabinose,and a small amount of fucose.There are great differences in the proportion of monosaccharides in different flowering stages.The content of glucose tends to be stable from the beginning of the big white period.The content of galacturonic acid increased significantly from the early bud stage to the white flower stage.The content of galactose tends to be stable from the white flower stage.The content of xylose is highest in white flowering period.Arabinose content begins to decline from the big white period.The content of fucose is always much lower than other monosaccharides.The content of mannose and rhamnose did not increase significantly.Conclusion The established method has high sensitivity,fast analysis speed,good resolution.It can be used to determine Honeysuckle polysaccharide content and monosaccharide composition,and provide a reliable analytical method for the study of structure,pharmacological activity and quality of honeysuckle polysaccharide and other plant polysaccharides. |
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