Anti-Aging Effect Of Aqueous Extract From Astragali Radix And Its Mechanism On Drosophila Melanogaster

Rationales: Aging is an irresistible physiological change caused by tissue cell aging and organ function decline with age.The aging process is accompanied by neurodegenerative diseases,cardiovascular diseases,diabetes,cancer and many other diseases,so it is very important to delay the aging process,improve the quality of life and health of the elderly,and achieve healthy aging.Anti-aging Chinese medicine is very important in this respect.Astragali Radix is the dry root of the leguminous plants Astragalus membranaceus(Fisch.)Bge.Var.mongholicus(Bge.)Hsiao and Astragalus membranaceus(Fisch.)Bge.The main components of astragalus are polysaccharide,brass,saponins and so on.More and more pharmacology shows that astragalus extract can increase telomerase activity,anti-oxidation,anti-inflammatory,immune regulation,anti-cancer,lowering blood lipid,lowering blood sugar,liver protection,expectorant,diuretic and other effects.Drosophila melanogaster has been widely used in the study of anti-aging drugs and due to its advantages of easy feeding,short growth cycle,strong reproductive capacity,easy discrimination between male and female,and mature genetic manipulation.Astragalus is mostly used as decoction in clinical use.In this paper,we studied the anti-aging effect of aqueous extract from Astragali Radix on Drosophila and its mechanism.Objective: To clarify the anti-aging pharmacodynamic evaluation of aqueous extract from Astragali Radix in Drosophila and its mechanism.Methods: 1.Study on the pharmacodynamics of aqueous extracts of Astragali Radix(AEAR)to retard the aging of Drosophila.In order to investigate the effect of AEAR on the life span of natural aging Drosophila,flies were cultured with the common food containing different concentration of AEAR(0.625 mg/m L,1.25 mg/m L,2.5 mg/m L,5 mg/m L,10 mg/m L and15 mg/m L)at different administration times(drug treatment from15 to 35 days,drug treatment from 30 to 45 days,drug treatment in the whole life cycle).Flies were fed with common food only in the control group.On thebasis of the optimal dose selected from the lifespan experiment,the influence of AEAR on the climbing ability and food intake of Drosophila was investigated,and the effect of AEAR on the life span of Drosophila was analyzed by H2O2 damage model and paraquat damage model.2.Study on the mechanism of AEAR to delay the aging of Drosophila.The metabolite changes of Drosophila in the different groups(3 d control group,20 d aging group and 20 d aging group + AEAR)were studied by using UHPLC-MS/MS metabolomics technology.The antioxidant activity of AEAR against DPPH in vitro was determined,and the activity of antioxidant enzymes SOD and CAT in Drosophila,as well as the m RNA expression levels of antioxidase-related genes SOD1,SOD2,SOD3 and CAT in Drosophila were tested.In this way,Based on the above research,the mechanism of AEAR to delay the aging of Drosophila was clarified.Reasults: 1.In the process of delaying Drosophila aging,AEAR is not suitable for administration in the whole life cycle.In the young period(15-35days),the effective dose of AEAR to delay the aging of male Drosophila is1.25 mg/m L,and the effective dose of AEAR to delay the aging of female Drosophila is 0.625 mg/m L.When administered in the aged period(30-45days),the effective doses of AEAR to delay the aging of male Drosophila were 1.25 mg/m L,2.5 mg/m L and 5 mg/m L,and the effective doses of AEAR to delay the aging of female Drosophila were 1.25 mg/m L and 2.5mg/m L.At the same time,on the basis of the optimal dose,AEAR could improve the climbing ability and food intake of Drosophila,and expand the lifespan of the H2O2 damage model and the paraquat damage model of Drosophila.2.The metabolite changes of Drosophila in the different groups(3 d control group,20 d aging group and 20 d aging group + AEAR)were studied by using UHPLC-MS/MS metabolomics technology.The results showed that8 aging-related differential metabolites were identified,including DL-lysine,Histamine,D-mannose-6-phosphate,L-glutamic acid,nicotinic acid,citric acid,pantothenic acid(vitamin B5),talinolol.These 8 metabolites are mainlyinvolved in amino acid metabolism and glucose metabolism(D-glutamine and D-glutamate metabolism,alanine,aspartic acid and glutamate metabolism,fructose and mannose metabolism,amino acids and nucleotides metabolism,arginine and proline metabolism.Meanwhile,the AEAR showed a dose-dependent effect on the scavenging ability of DPPH in vitro,and the SOD and CAT enzyme activity in the aging group also increased afetr the intervention of AEARConclusion: AEAR can significantly delay the aging of Drosophila,improve the climbing ability and food intake of Drosophila,and increase the activity of antioxidant enzymes in Drosophila.Therefore,the anti-aging mechanism of AEAR on Drosophila may be related to the improvement of antioxidant capacity of the body.