Studies On Chemical Constituents Of Patrinia Scabiosaefolia

Patrinia scabiosaefolia Fisch.,also known as"Huanghualongya",is one of the sources of traditional Chinese medicine"baijiangcao",which has the effects ofheat-clearing and detoxifying,reducing swelling and expel pus,and promoting blood circulation for removing blood stasis,expecially in chronic appendicitis.In this article,the chemical constituents of Patrinia scabiosaefolia Fisch.are studied for the sake of enriching the information in chemical composition and acquiring the ingredients with novel structure and significant activity,which is expected to provide basic data for the quality standard?mixed product problem?,bioactive components,clinical application and the development and utilization of Patrinia scabiosaefolia Fisch.The chemical constituents from the 95%ethanol extract of Patrinia scabiosaefoliaFisch.are separated and puified by using kinds of modern chromatographic technique-s,such as silica gel,Sephadex LH-20,MCI,ODS,PHPLC and MPLC.The structures of isolated compounds was elucidated according to their data of UV,IR,HR-ESI-MS,NMR,etc.Further,the cytoxic activity of the isolated compounds against human color-ectal carcinoma?HCT-116?was investigated by CCK-8 method.58 compounds were isolated from the CHCl₂and Et OAc sections of Patriniascabiosaefolia Fisch.including 4 novel compounds?1,17,20,27,Figure I?and 1new natural compund?57?.The absolute configuration of 1 was determined by CD.These compounds are 16 lignan compounds?1-16?:Patrinianeolignan I?1?,Isodonoside VI?2?,?7S,8R?dihydrodehydrodiconiferyl alcohol 4-O-?-D-glucopyranoside?3?,?+?-Nortrachelogenin?4?,Wasabisides A?5?,isolariciresinol?6?,?-?-isolariciresinol 4-?-D-glucopyranoside?7?,?8S,7'R,8'S?-Isolarisiresinol 9'-O-?-D-glucopyranoside?8?,?8R,7'S,8'R?-Isolarisiresinol 9'-O-?-D-glucopyranoside?9?,epipinoresinol?10?,syringaresinol?11?,eudesmin?12?,pinoresinol?13?,medioresinol?14?,?-?-?7R,7'R,7''S,8S,8'S,8''S?-4,4''-Dihydroxy-3',3,3'',5'-tetramethoxy-7,9':7',9-diepoxy-4',8''-oxy-8,8'-sesquineolignan-7'',9''diol?15?,Salicifoliol?16?;10 monoterpenoids?17-26?:6,7-Dehydrodissectol A?17?,Dissectol A?18?,2,6-dimethyloctane-1,8-diol?19?,Patriniaol A?20?,Isoboonein?21?,?4R,5R,7S,8S,9S?-7-hydroxy-8-hydroxymethyl-4-methyl-perhydrocyclopenta[c]pyran-1-one?22?,Patrinoside?23?,sweroside?24?,7Z-roseoside?25?,3,2',3',4',6'-penta-O-acetyl-?3R,9R?-3-hydroxy 7,8-didehydro-?-ionol 9-O-?-D-glucopyranoside?26?;3 pyrones?27-29?:Hydroxymaltol 3-O-?6'-O-trans-caffeoyl?-?-D-glucopyranoside?27?,Bockioside A?28?,Pyromeconic acid 3-O-?-D-glucopyranoside 6'-?O-4''-hydroxy-benzoate??29?;8 simple Phenylpropanoids?30-37?:Convoldorine?30?,3-O-p-coumaroylquinic acid?31?,coniferaldehyde?32?,sinapaldehyde?33?,C-Veratroylglycol?34?,2,3-dihydroxy-1-?4-hydroxy-3,5-dimethoxyphenyl?-1-propanone?35?,Sawaranospirolide?36?,Cyclospirolide?37?;3coumarin compounds?38-40?:scopoletin?38?,7-Methoxycoumarin-6-?-D-glucopyranoside?39?,aseculin?40?;2 phenylmethanol glycosides?41-42?:benzyl-O-?-D-glucopyranoside?41?,Icariside F2?42?;2 phenylethanoid glycosides?43-44?:2-phenemyl-1-O-?-D-glucopyranoside?43?,Icariside D1?44?;3 alkaloids?45-47?:Cytochalasin D?45?,berberine?46?,1H-indole-3-carbaldehyde?47?;2 flavonoid glycosides?48-49?:Clovin?48?,Robinin?49?;1 anthraquinone:Emodin?50?;1 steroid:?-Sitosterol?51?;1 triterpenoid:ursolic acid?52?;6 other compounds?53-58?:2?,6?-dihydroxy-5-[?E?-3'-hydroxy-3'-methyl-1'-butenyl]-6-methyl-4-cyclohexen-3-one?53?,Loliolide?54?,Isololiolide?55?,3,4,5-trimethoxyphenyl-1-?-D-glucopyranoside?56?,8,9-didehydro-7-hydroxydolichodial?57?,p-hydroxybenzaldehyde?58?.All of the isolates were evaluated in vitro for anti-colorectal cancer at 100 u M.Among these isolated compounds,compounds 12,20 and 46 exhibited mediun antitumor activity against HCT-116 with IC₅₀values of 41.92u M,42.23u M and117.2u M,respectively.