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Preparation And Related Properties Studies Of Paclitaxel Loaded And Her-2-targeted PLGA-COOH Nanoparticle Ultrasound Contrast Agent

Posted on:2019-06-14Degree:MasterType:Thesis
Country:ChinaCandidate:Q WenFull Text:PDF
GTID:2404330647964432Subject:Clinical medicine
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Objective To prepare a polymer ultrasound contrast agent carrying paclitaxel and anti HER-2 antibody.To check its general characteristics,in vitro imaging,and to investigate its effect on the proliferation and apoptosis of breast cancer cells in vitro.Method The paclitaxel loaded and anti HER-2 antibodies polymer ultrasound contrast agents were produced by the double emulsion technique and carbodiimide technique.Testing its general properties.The effect of imaging in vitro was observed by the high frequency ultrasonography.Observing the combination of drug-loading targeted contrast agent and SKBR-3 breast cancer cells,and to observe the proliferation and apoptosis of breast cancer cells play a role in promoting.Result The average diameter of Drug-loaded targeting PLGA nano ultrasound contrast agent was(318.07 ± 12.51)nm,the surface potential was(-2.16 ± 0.31)m V,the drug encapsulation efficiency was(60.00± 3.40)%,the drug loading amounts was(6.00 ± 0.34)%,the contrast agent was spherical particles,uniform size,good dispersion.Inverted fluorescence microscope and laser confocal microscope found the combination of anti HER-2 antibody and PLGA-COOH polymer contrast agent is large,flow cytometry observed the combination rate is(99.59± 0.42)%.In vitro imaging experiments showed that drug targeting macromclecular imgaging agent showed uniform and fine dotted dense high echo.Search target experiment in vitro observed drug targeting macromolecular contrast agent and SBKR-3 breast cancer cells have a stronger binding rate,The drug targeting contrast agent promoted breast cancer cell apoptosis.Conclusio To prepare successfully a kind of her-2-targeted and paclitaxel loaded high molecular contrast agent.The external imaging is better.It and SKBR-3 cells have the high binding rate,it also played a role for apotosis of breast cancer.
Keywords/Search Tags:Polymer ultrasound contrast agent, Anti HER-2 antibody, Paclitaxel, Breast cancer cell
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