Impact Of Short-term Hypoxia Trainingon Intestinal Inflammation Factors And Related Indexes Such As TLR4 In Rats

Objective: Hypoxia exposure and exercise have been found to regulate intestinal inflammation,while the combined effect of them on intestinal inflammation factors and related indexes such as TLR4 have not been well understood.In this study,120 rats were subjected to hypoxia and exercise intervention.Intestinal and blood-related inflammatory markers were used to investigate the impact of short-term hypoxia exposure and hypoxia training on intestinal inflammation factors and related indexes such as TLR4,which helps understand the changes of intestinal inflammation factors and related indexes in hypoxic training,with a view to provide new ideas for preventing the occurrence of intestinal inflammation in hypoxia training.Methods: 120 rats were divided into 12 groups according to both four kinds of intervention: normoxia resting,normoxia training(treadmill,24 m / min,45 min / d),hypoxia resting(oxygen content of 12.8%,simulated 4000 m normobaric hypoxia),hypoxia training,and three drawing time points(3,6,9 days,24 h after exercise groups were trained).The expression of TLR4 m RNA,protein expression of NF-κB,IL-6 and claudin-1 in the gut,the concentrations of IL-6 in serum,and the changes of WBC in the blood were analyzed by ELISA and molecular biological experimental methods of RT-PCR and Western Blot after the intervention of each drawing time point.Results:(1)At 3rd day,WBC and Mon% were significantly increased in HR(P<.01).WBC was significantly increased in HE.(2)At 6th day,the expression of TLR4 m RNA in intestinal of HR and HE were significantly lower than that in NR(P <0.01),while TLR4 m RNA in HE was lower than that of NE(P <0.05).Furthermore,NF-κB protein in the intestine of HR was significantly higher than that in NR(P <0.05),while the concentrations of IL-6 in serum of NE and HE were significantly lower than those in NR(P<.05).And the level of IL-6 in HE was significantly lower than that in HR(P <.05).(3)At 9th day,NF-κB protein in the gut of HE was significantly higher than that of NE,while the level of IL-6 in serum of HE was significantly lower than that of NR and HR(P <.05,P<.01).Conclusion:(1)Short-term hypoxia exposure and hypoxia training(simulated 4000 m normobaric hypoxia)could cause the down-regulation of TLR4 m RNA and increase the expression of NF-κB protein,but did not cause significant intestinal inflammation.(2)Short-term hypoxia exposure caused significant changes in intestinal TLR4 m RNA and NF-κB protein at 6th days and turned normal at 9th day.(3)IL-6 in serum could not be used to reflect the changes of inflammatory cytokine IL-6 in the intestine.