| Objective: With the improvement of human living standards and the satisfaction of material needs,obesity has begun to spread globally.As a safe and effective weight loss method,exercise has been accepted by more and more people.High intensity interval training has been promoted in recent years because it can reduce more weight.UCP-1 is the core protein of fat burning and heat production,and is also regarded as a marker of "white adipose browning".The purpose of this study was to find out whether high intensity interval training can activate the expression of UCP-1 in fat by generating stronger muscle factors,triggering more immune regulation or sympathetic regulation than moderate intensity continuous training to achieve weight loss,providing theoretical basis and experiment evidence for enriching exercise prescription.Research materials and methods: 70 C57BL/6J female mice weighing 3.67±1.12 g age d at 3 weeks were selected.Mice were randomly divided into a normal fat diet group(10 in the NFD group)and a high fat diet group(60 in the HFD group).The body weight of the mice was recorded every week.When the weight of the mice in the HFD group exceeded 110% of the average weight of the NFD group,the obesity model was successfully established.Then the obese mice were randomly divided into high intensity interval training group(10 in the HIIT group),moderate intensity continuous training group(10 in the MICT group),and the high fat diet group without exercise(10 in the HFD group).The training program of the MICT group was 60% of the running speed of the mouse under the maximum exercise capacity and runs for 45 minutes.The training program of the HIIT group was running for 1 minute under the maximum speed,and then running at 60% maximum speed for 2 minutes.Perform to ensure that the motion distances of the two exercise groups were equal.In order to ensure the accuracy of exercise intensity,the maximum running speed of the mice was measured by an incremental load test every two weeks.The frequency of exercise was 5 days/week and lasted for 12 weeks.During the experiment,the food intake and body weight of the mice were recorded weekly,and take the samples 48 hours later after the last exercise.Colorimetric assay for triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),and Western Blot immunoblotting for detection of gastrocnemius PGC-1?,FNDC5 protein expression levels,UCP-1,TH and IL-6 protein expression levels in the inguinal subcutaneous fat and visceral fat around the uterus.The body weight and total food intake were recorded,and the nose tip of the mouse was measured to the length of the anus segment,and the Lee's index was calculated in combination with the body weight.Results:?There was no significant difference in total food intake between the three groups(p>0.05).Compared with HFD group,the body weights of two exercise intervention groups were significantly reduced(p<0.01),while there was no significant difference between the HIIT group and MICT group(p>0.05).Compared with HFD group and MICT group,the weight of visceral fat around the uterus of HIIT group was significantly reduced(p<0.01).Compared with HFD group,the weight of subcutaneous fat in the inguinal of HIIT group was significantly reduced(p<0.01)as well,while that of MICT group was reduced too,but there was no significant difference(p=0.076).In addition,compared with HFD group,the Lee's index of HFD group was significantly reduced(p<0.05)while there was no significant change between any other groups(p>0.05).?There was no significant difference in HDL-C,TG and TC between the serum of all mice(p>0.05),while the LDL-C of the HIIT group was significantly lower than that of the HFD group(p<0.01),significantly lower than the MICT group(p<0.05).LDL-C of MICT group mice was significantly lower than HFD group(p<0.05).?The relative expression of PGC-1? protein in the gastrocnemius muscle of the HIIT group was significantly higher than that of the HFD group(p<0.05).There was no significant difference in the relative expression of FNDC5 protein in the gastrocnemius muscle of the three groups(p>0.05).?The relative expression of UCP-1 protein in the peri-uterine fat of the HIIT group was significantly higher than that of the HFD group and the MICT group(p<0.05).There was no significant difference in the relative expression of UCP-1 protein in the subcutaneous fat of the three groups of mice(p>0.05).?The relative expression of TH protein in the uterus of the HIIT group was significantly higher than that of the MICT group(p<0.01),and the data of the HFD group was significantly higher than that of the MICT group(p<0.05).The relative expression of TH protein in the inguinal subcutaneous fat of the HIIT group was significantly higher than that of the MICT group(p<0.01),and significantly higher than that of the HFD group(p<0.05).?The relative expression of IL-6 protein in the peri-uterine fat of mice in group T was significantly higher than that in group M(p<0.01).The relative expression of IL-6 protein in the peri-uterine fat of group M was higher than that in group H(p=0.087).There was no significant difference in the relative expression of IL-6 protein in the subcutaneous fat of the mouse inguinal region(p>0.05).Conclusion: Both 12-week HIIT and MICT were effective in reducing the body weight of obese mice,and HIIT was more effective in reducing visceral fat weight.In visceral adipose tissue,HIIT can produce more IL-6 and TH than MICT.It is speculated that HIIT activates UCP-1 by enhancing sympathetic nerves and stimulating the immune system to improve the oxidation of visceral adipose tissue. |
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