Effect Of Aerobic Exercise Combined With Oyster Peptide On Sarcopenia Formation In SHR Rats And Its Mechanism

Objective:To explore the intervention effect,interaction and physiological mechanism of aerobic exercise and oyster peptide supplementation on the formation of Sarcopenia in spontaneous hypertensive rats(SHR),which is the primary hypertension and its non-drugs causing Sarcopenia The treatment method provides the necessary experimental basis.Method;Five week old SPF male 10 WKY rats and 40 SHR were selected.After one week of adaptation,40 SHRs were randomly divided into SHR control group(SC group,N=10),aerobic exercise group(AE group,N=10),oyster peptide group(OP group,N=10),and aerobic Exercise+oyster peptide group(AEOP group,N=10)and WKY control group(WC group,N=10).The SC,WC,and OP groups usually did not exercise;rats in AE,AEOP group underwent 60 min weight-free swimming training at a fixed time every afternoon;oyster peptide was administered every morning with a supplementary dose of 1.2 g/Kg body weight.Weigh your weight on time every week and measure your blood pressure every two weeks.After 8 weeks,by observing the morphological structure of skeletal muscle,the total protein,TNF-? content,and Ub,MuRF-1 and MAFbx mRNA expression levels of skeletal muscle in each group were detected.To explore the intervention effect and mechanism of aerobic exercise combined with oyster peptides on Sarcopenia caused by hypertension.Results:(1)Compared with the WC group,the systolic and diastolic blood pressures of the SC rats were significantly increased(P<0.01,P<0.01),and the skeletal muscle weight and SI ratio were significantly reduced(P<0.05,P<0.01).The results of HE staining showed that the skeletal muscle fibers in the SC group were disordered,showing that the muscle fibers were rounded,the cross-sectional area of the skeletal muscle was small,and the nucleus centered.In the WC group,the cross-sectional area of skeletal muscles was larger,the skeletal muscle fibers were arranged more neatly,the boundaries of muscle cells were clearer,the cross sections of muscle cells were polygonal,and the nucleus was at the cell edge.(2)Compared with the WC group,the total protein content of skeletal muscle in SC group was significantly reduced(P<0.01);the TNF-? content of skeletal muscle was significantly increased(P<0.01);Ub and MuRF-1 mRNA in skeletal muscle The expression level was significantly increased(P<0.05);although the expression level of skeletal muscle MAFbx mRNA was increased,there was no significant difference(P>0.05).(3)The results of two-factor analysis of variance show that aerobic exercise combined with oyster peptide supplementation has a very significant interactive effect on reducing SHR systolic and diastolic blood pressure(F(1,37)=122.658,P=0.000,F(1,37)=64.690,P=0.000);there is no significant interaction effect on the changes of SHR skeletal muscle weight,body weight and SI ratio(F(1,37)=0.734,P=0.398;F(1,37)=0.025,P=0.875;F(1,37)=2.705,P=0.109).Aerobic exercise has a very significant effect on reducing SHR systolic and diastolic blood pressure F(1,37)=186.862,P=0.000:F(1,37)=67.043,P=0.000);on SHR skeletal muscle weight Decreased,and increased the SHR SI ratio,but there was no significant difference(F(1,37)=2.783,P=0.104;F(1,37)=0.34,P=0.796).Body weight has a significant effect(F(1,37)=23.595,P=0.000).Oyster peptide supplementation has a very significant effect on reducing SHR systolic and diastolic blood pressure(F(1,37)=130.321,P=0.000;F(1,37)=47.385,P=0.000);although it has Reduced,but no significant difference(F(1,37)=3.849,P=0.058);have significant effects on increasing RHR weight and increasing SHR SI ratio(F(1,37)=5.287,P=0.028:F(1,37)=6.489,P=0.016).(4)The results of two-factor analysis of variance showed that aerobic exercise combined with oyster peptide supplementation had no significant interaction effects on the total protein content of SHR skeletal muscle and TNF-? content of skeletal muscle,skeletal muscle Ub,MAFbx and MuRF-lmRNA expression levels(F(1,37)=3.023,P=0.0.091,F(1,37)=2.845,P=0.101;F(1,37)=3.085,P=0.090;F(1,37)=0.829,P=0.369;F(1,37)=0,708,P=0.406).Aerobic exercise has a significant effect on increasing the total protein content of SHR skeletal muscle(F(1,37)=4.838,P=0.035);it has a significant effect on reducing the content of TNF-? in SHR skeletal muscle(F(1,37)=7.204,P=0.011);has a significant effect on reducing the expression levels of SHR skeletal muscle Ub,MAFbx and MuRF-1 mRNA(F(1,37)=4.349,P=0.047;F(1,37)=9.456,P=0.004,F(1,37)=9.456,P=0.004).Oyster peptide supplementation had a very significant effect on increasing the total protein content of SHR skeletal muscle(F(1,37)=8.909,P=0.005);although the content of TNF-?in SHR skeletal muscle was reduced,there was no significant effect(F(1,37)=1.368,P=0.250);There is no sinificant effect on reducing the expression levels of SHR skeletal muscle Ub,MAFbx and MuRF-lmRNA(F(1,37)=0.784,P=0.384;F(1,37)=2.073,P=0.160;F(1,37)=0.361,P=0.076).Conclusion:(1)SHR significantly increased systolic and diastolic blood pressure,increased TNF-?content in skeletal muscle and significantly up-regulated Ub,MuRF1 and MAFbx mRNA expression in skeletal muscle,leading to a reduction in total protein content in skeletal muscle.Therefore,due to elevated blood pressure,activation of the TNF-?-UPP signaling pathway in skeletal muscles causes an increase in skeletal muscle protein breakdown,leading to Sarcopenia.(2)Aerobic exercise and oyster peptide supplementation can effectively reduce the systolic and diastolic blood pressure of SHR.Aerobic exercise can significantly reduce the TNF-? content of SHR skeletal muscle,inhibit the expression of Ub,MuRF1 and MAFbx mRNA in skeletal muscle,and increase bone Total muscle protein content,thus preventing Sarcopenia.Oyster peptides can significantly increase the total protein content of skeletal muscle,but supplementation of oyster peptides may not prevent the occurrence of Sarcopenia by inhibiting the activation of the TNF-?-UPP signaling pathway in skeletal muscle.