| Objective: skeletal muscle injury is a common injury in sports injury,and its treatment has always been a research hotspot.The purpose of this study is to investigate the effect of sarcoidan polysaccharide on the healing process of skeletal muscle in rats.Methods: 24 8-week-old Wistar healthy male rats were randomly divided into 4 groups,6 rats in each group.Groups: quiet control group(A),centrifugal injury group(B),natural recovery group(C),coralline polysaccharide intervention group(D).The model of sports injury was established by high-intensity centrifugal exercise,and the time and gradient of each week increased continuously in four weeks.After the model was established successfully,the rats were given gavage for one week and recovered naturally.Pentobarbital sodium was used for anesthesia and intraperitoneal injection.Take blood from abdominal aorta,centrifugate it to obtain serum and store it in ultra-low temperature refrigerator;quickly take a small amount of soleus muscle and put it into polyformaldehyde for fixation;take soleus muscle,such as rice grain size,and quickly put it into 3% glutaraldehyde fixative solution for fixation;then take soleus muscle and quickly put it into EP tube tin paper and wrap it in ultra-low temperature refrigerator to wait for homogenization.(1)Serum CK,LDH,MDA and T-AOC were detected by colorimetry.(2)The m RNA of miR-155-5p,miR-133a-5p,SOCS1 m RNA and IL-1 ? were detected by RT-PCR.(3)The expression of SOCS1 in skeletal muscle was detected by immunohistochemistry and Western blot.(4)The expression of Pax7 protein in skeletal muscle was detected by immunofluorescence.(5)The morphology of skeletal muscle was observed.Results:1.Compared with group A,the level of serum CK and LDH in group B increased significantly(P < 0.01).After recovery,compared with group A,the level of CK and LDH in group C was still higher(P < 0.05 or P < 0.01),and there was no difference between group D and group A(P > 0.05).2.Compared with group A,the level of MDA in group B was significantly higher after injury(P < 0.01).The level of MDA in group C was still higher than that in group A(P < 0.05),and there was no difference between group D and group A(P > 0.05).There was no significant difference in the content of T-AOC(P > 0.05).3.Compared with group A,the expression level of mir-155-5p in skeletal muscle of group B was significantly higher after exercise(P < 0.01).After recovery,the content of mir-155-5p in skeletal muscle of group C was still higher than that of group A(P < 0.01),the difference was very significant,and the difference between group D and group a decreased(P < 0.05).There was no significant difference in the expression of mir-133a-5p.3.Compared with group A,the expression level of mir-155-5p in skeletal muscle of group B increased significantly(P < 0.01).After recovery,the expression level of mir-155-5p in skeletal muscle of group C was higher than that of group A(P < 0.01),and the difference between group D and group A was significant(P < 0.05).There was no significant difference in the expression of mir-133a-5p(P > 0.05).4.Compared with group A,the expression level of socs1 mrna and SOCS1 protein in group B was significantly higher(P < 0.01).After recovery,the expression level of socs1 mrna and SOCS1 protein in group C was higher than that in group A(P < 0.05 or P < 0.01),and that in group D was significantly higher than that in group A,B and C(P < 0.01).5.Compared with group A,the expression level of IL-1 ? m RNA in skeletal muscle of group B after injury was significantly higher(P < 0.01),with statistical significance;after recovery,compared with group A,the expression level of IL-1 ? m RNA in skeletal muscle of group C was still higher,with significant difference(P < 0.01).The expression level of IL-1 ? m RNA in skeletal muscle of group D was lower than that of group A(P > 0.05).6.Compared with group A,the expression level of Pax7 protein in group B increased significantly(P < 0.01).After recovery,the expression level of Pax7 protein in group C was still higher than that in group A(P < 0.01),and that in group D was still higher than that in group A(P < 0.01),and that in group D was still higher than that in group B(P < 0.01).The difference between group D and group C was significant(P < 0.05).7.Through electron microscopy,it was found that the sarcomere and Z-line of skeletal muscle in group A were arranged in order,and the mitochondria were arranged on both sides of Z-line,with uniform size.Compared with group A,sarcomere of group B was distorted,large area of myofilament was broken,structure was disordered and mitochondria were swollen.In group C,the Z line is still in fracture state.In group D,the Z lines were arranged clearly and neatly.Sarcomere is not broken or twisted,and mitochondria are arranged orderly.Conclusion: 1.The skeletal muscle injury model of Wistar male rats can be effectively established by continuous high-intensity centrifugal exercise.2.After skeletal muscle injury,the contents of CK,LDH and MDA increased significantly,and the expression levels of mir-155-5p,IL-1 ? m RNA,SOCS1,socs1 mrna and Pax7 increased significantly.Under electron microscope,the skeletal muscle fibers were obviously damaged;3.after the intervention of sarcoidosis polysaccharide,the contents of CK,LDH and MDA were significantly reduced,the expression levels of mir-155-5p,IL-1 ? m RNA and Pax7 were significantly reduced,and the expression levels of SOCS1 and socs1 mrna were further increased.The recovery of muscle fiber was obvious after the intervention of sarcoidosis polysaccharide.4.Sarcoidosis polysaccharide has a certain effect on the treatment of exercise-induced skeletal muscle injury,and its mechanism may be related to mir-155-5p/SOCS1/IL-1 ? signal pathway. |
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