| Capillary electrophoresisis a separation technology combinedelectrophoresis with chromatography theory,HPCE has the avantages of high efficiency,short analysis time,variousseparationmodes,high degree of' automation and micro sampling.Widelyusedintheareaofseparationandanalysis.In this peper,high performance capillary electrophoresissaparation and analysis of active ingredient and ennzyme reaction kinetics were brieflyreviewed in recent years.And on this basis,three simultaneous separation and analysis methodof biological active substances and onestudy on ennzyme reaction kinetics of high performance capillary electrophoresismothed has been estabilished.The specific contents of the paper are as follows:1.A new micelle capillary electrophoresis method was developed for simultaneous separation and determination of hanfangchin A,hanfangchin B arid Berbamine hydrochloride.The three compounds could be effectively separated and determined within 25 mins in a running buffer(pH 4.09)consisting of 38 mmol/L NaH2PO4,5 mmol/L ?-CD,19 mmol/L SDS,33%(v/v)acetonitrile and 0.3%(v/v)etrahydrofuranwith the applied voltage of 25 kV,capillary temperature of 25 ?,detector wavelength of 230 nm,and injection of 5 s at 0.5.psi.The linear range for the determination of hanfangchin A,hanfangchin B and Berbamine hydrochloride were 3.0?120.0,5.0?100.0 and 10.0?100.0 ?g/mL,respectively.The proposed method has been successfully used for determination of the three constituents in different samples with the recovery ranged between 96.8%and 104.2%,and the relative standard deviation of below 4.0%.2.A new capillary zone electrophoresis method for simultaneous separation and determination ofvinblastine,vincristine,catharanthine and vdoline.The fore compounds could be effectively separated and determined within 14 mins in a running buffer(pH 4.31)consisting of 39 mmol/L NaH2PO4,2.0%(v/v)n-butyl alcohol and 10%(v/v)acetonitrile.with the applied voltage of 20 kV,capillary temperature of 25 ?,detector wavelength of 214 nm,and injection of 5 s at 0.5 psi.The linear range were 5.0?150.0?5.0?150.0?2.5?150.0 and 5.0?250 ?g/mL.The proposed method has been successfully used for determination of the fore constituents in different samples with the recovery rangedbetween 93.7%and 108.9%,and the relative standard deviation of below 4.0%.3.A new capillary zone electrophoresis method usingthree ethanol amine as organic additives for separation and determination of neochlorogenic acid,cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid A,1,5-dicaffeoylquinic acid,isochlorogenic acid C,isochlorogenic acid B and 1,3-dicaffeoylquinic acid.The eightcompounds could be separated and determined in arunning buffer(pH 8.64)consisting of 30 mmol/L Na2B4O7,10 mmol/L NaH2PO4,1.2%(v/v)three ethanol amine,0.3%(v/v)etrahydrofuranand 2.0%(v/v)isopropyl alcohol with the applied voltage of 20 kV,capillary temperature of 25 ?,detector wavelength of 326 nm,and injection of 3 s at 0.5 psi.The linear range were 5.0?100.0?5.0?120.0?2.5?180.0?5.0?160.0?5.0?180.0?5.0?120.0?5.0?120.0?5.0?180.0 ?g/mL.The proposed method has been successfully used for determination of the eight constituents in different samples with the recovery ranged between 92.6%and 106.8%,and the relative standard deviation of below 5.0%.4.A new a-glucosidase inhibitorinvitroscreeningmethods has developed.using PNPG as substrate,PNPG and the product(PNP)could be separated and determined in a running buffer(pH 9.36)consisting of 30 mmol/L Na2B4O7 and 4 mmol/L NaOH with the applied voltage of 25 kV,capillary temperature of 25 ?,detector wavelength of 314 nm,and injection of 5 s at 0.5 psi.The proposed method has been successfully used for detecting inhibitory activity of Luteolin,Curcumin,Ginkgo biloba and onion extract.with the IC50 were 11.96 ?g/mL,51.44 ?g/mL,4.27 mg/mL and 8.17 mg/mL.Curcumin and Ginkgo were competitive type,inhibition constants were 1.41 mg/L ? 1.72 mg/L... |
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