Research On The Separation And Analysis Of Several Types Of Active Ingredients Of Traditional Chinese Medicine By High Performance Liquid Chromatography

HPLC(High performance liquid chromatography)has the advantages of versatile application,high separation efficiency,high analysis velocity,high sensitivity and wide mobile phase choices.In this paper,separating and analyzing of active ingredient in some herbs by HPLC were briefly reviewed.And five simultaneous separation and analysis method of the active substances have been established.The specific contents of the paper are as follows:1.After optimizing mobile phase and gradient elution procedure,a new quick method for simultaneous separation and determination of hydroxyl safflower A,crocin-? and crocin-? using methanol-0.3%(v/v)phosphoric acid solution as the mobile phase was developed.The three compounds could be effectively separated and determined within 10 mins.The linear range for the determination of hydroxyl safflower A,crocin-? and crocin-? were 1.2~80?1.88~62.7 and 1.76~176?g/mL,respectively.The proposed method has been successfully used for determination of the three constituents in HongHua RuYi pills with the recovery ranged between 98.9% and 103.6%,and the relative standard deviation of below 2%.2.A new method for simultaneous separation and determination of schisandrol B,schisantherin A,schisanhenol,schizandrin B,schizandrin A,and schizandrin C was developed after optimizing the condition of HPLC,and the six active ingredient were determined simultaneously by HPLC using acetonitrile-water as mobile phase for gradient elution.The six compounds could be effectively separated and determined within 17 mins.The linear range for the determination of schisandrol B,schisantherin A,schisanhenol,schizandrin B,schizandrin A,and schizandrin C were 1.36~174.3?2.46~314.3?1.29~165.7?1.54~197.1?1.15~147.1 and 1.32~168.6?g/mL,respectively.The proposed method has been successfully used for determination of the six constituents in fructus schisandrae chinensis with the recovery ranged between 96.8% and 100.5%,and the relative standard deviation of below 1%.3.Using acetonitrile-0.1%(v/v)phosphoric acid solution as mobile phase for elution,a quick,sensitive method for simultaneous separation and determination of liquiritin,isoliquiritin,liquiritigenin,isoliquiritigenin,licochalcone A,glabridin and glcyrrhetinic acid in glycyrrhiza was developed.And to solve the problem of different max absorption wavelength for the seven ingredient in glycyrrhiza,HPLC wavelength switching method was used.The seven compounds could be effectively separated and determined within 23 mins.The linear range for the determination of liquiritin,isoliquiritin,liquiritigenin,isoliquiritigenin,licochalcone A,glabridin and glcyrrhetinic acid were 0.967~145.0?0.51~127.5?0.55~137.5?0.933~140?1.074~137.5 ?0.52 ~ 130 and 0.977 ~ 125?g/mL,respectively.The proposed method has been successfully used for determination of the seven constituents in glycyrrhiza with the recovery ranged between 96.0% and 102.8%,and the relative standard deviation of below 2.5%.4.A new method for simultaneous separation and determination of vdoline,vincristine,catharanthine,and vinblastine was developed after optimizing the condition of HPLC.The mobile phase was simple,the analysis time was short and the linear characteristic was fine.The four compounds could be effectively separated and determined within 15 mins using acetonitrile-0.2%(v/v)diethylamine solution as the mobile phase for gradient elution.The linear range for the determination of vdoline,vincristine,catharanthine,and vinblastine were 1.719~220?1.292~248?1.25~240 and 1.083~208 ?g/mL,respectively.The proposed method has been successfully used for determination of the four constituents in catharanthus roseus with the recovery ranged between 95.6% and 101.8%,and the relative standard deviation of below 2%.5.A method for the determination of the new poisonous component dihydroxy decatenate in nutlet,shuck,deep-fried dough cake,branches and leaves of jatropha curcas by HPLC was developed,and the extractive method of the samples was researched.The sample was prepared using methanol and heating reflux for 2 hours,and the HPLC mobile phase was methanol-water(80:20).The linear range for the determination of the target component was 13.65~327.60 ?g/mL.Through analyzing the content of dihydroxy decatenate in nutlet,shuck,deep-fried dough cake,branches and leaves of jatropha curcas,it was concluded that the highest part was shuck.