| With the continuous development of the Bone Tissue Engineering,more and more bone bioremediation materials have attracted much attention.Because of good bioco MPatibility of bioactive ceramic materials,they can maintain their original nature in the body for a long time.At the same time,the implantation of the material has no toxic for body and local parts of the patient.They has sufficient biological strength to satisfy the demand for hard tissues of human bones.Such as hydroxyapatite whiskers,but its degradation rate has always hampered its development.Organic polymer materials have good physical mechanical properties and controllability of degradation rate,making it a good application prospect.Therefore,the experiment combines their advantages with hydroxyapatite whiskers(HAw),polycaprolactone(PCL),konjac glucomannan(KGM)as raw materials,by casting method,organic solvent evaporation method and freeze-drying.The PCL/HAw/KGM composite multi-layer porous scaffold with biomimetic human humerus was prepared and its surface morphology,composition,mechanical properties,pore size,porosity and related biological properties are detected and analyzed.The results are shown as follows.(1)Using polycaprolactone and hydroxyapatite whiskers as raw materials,Polycaprolactone/hydroxyapatite whisker composite film was successfully prepared by casting method and organic solvent evaporation method as a preparation method.The optimal experimental scheme obtained from the orthogonal experimental design.The optimum process conditions were that the amount of PCL added was 1.5 g,the amount of dichloromethane added was 15 m L,and the amount of HAw added was 0.05 g.The pore size distribution of PCL/HAw composite membrane prepared under the process parameters was mainly distributed in 5.893-14.898?m.The average pore size was 9.7515?m.The average tensile strength was 12.6573 MPa.The average elongation at break was 751.25%.The average contact angle was 82.998,less than 90° showed hydrophilicity.Cytotoxicity experiments showed that the degree of cell proliferation in 107-111%,so the cytotoxicity is 0 and no cytotoxicity.(2)Konjac glucomannan porous membrane was prepared by casting method and freeze-drying technique using konjac glucomannan as experimental material.In this paper,mechanical properties,porosity,degradation cycle,and pore size was chosen as evaluation indicators.The best cross-linking time was obtained by cross-linking the KGM porous membrane at different times(6h,12 h,and 18 h cross-linking).The results showed that: The konjac glucomannan porous membrane with alkali cross-linking for 12 hours has better comprehensive performance.The analysis shows that the pore size of konjac glucomannan porous membranes prepared by alkaline crosslinking for 12 h is mainly in the range of 50-400?m.The median value is about 150?m.The porosity is 96.4309%.The tensile strength is 2.5028 MPa,and the degradation cycle is 72 days.Cytotoxicity experiments showed that the degree of cell proliferation was distributed at about 110%,so the cytotoxicity was grade 0 and there was no cytotoxicity.(3)Preparation of PCL/HAw / KGM bionic composite multilayer porous scaffold by casting,organic solvent volatilization and freeze-drying using hydroxyapatite whiskers(HAw),polycaprolactone(PCL)and konjac glucomannan(KGM)as raw materials.Studies have shown that there are a large number of holes on the surface of the PCL/HAw/KGM biomimetic multi-layer porous scaffold.The connection between KGM porous membrane and PCL/HAw composite membrane is very tight.The porosity reaches 67.59%,and the pore size is mainly distributed in 100-400?m,the median value is about 150?m.After 60 days of degradation,the degradation rate reached 32.5%.Cytotoxicity experiments showed that the cell proliferation was distributed in 110%,so the cytotoxicity was 0,no cytotoxicity.The PCL/HAw/KGM bionic composite multi-layer porous scaffold prepared in this experiment can meet the basic requirements of bone tissue engineering for scaffold materials. |
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