| Celery is a biennial umbelliferous herb with a variety of biological activities,such as lowering blood pressure,lowering blood fat,and fighting antioxidants.The research on celery flavonoids mostly focuses on biological activity,and celery flavonoids have little research on digestion and absorption in vivo.In this paper,apiin was used as the research object,and the gastric absorption and intestinal perfusion models were used to study the mechanism of apiin absorption in vivo.Based on metabolomics techniques,liquid chromatography-mass spectrometry(LC/MS)was used to study the metabolic changes of apiin in vivo.The oxidative damage model of human umbilical vein cells(HUVECs)was used to investigate the changes of the activity factors of apiin metabolites on HUVECs and to evaluate the antioxidant capacity.It provides a theoretical basis for the rational diet of celery,the absorption and metabolism in the body and the functional activities of its metabolites.(1)Establishing a model of gastric and intestinal perfusion in rats,study the characteristics of apiin digestion and absorption in the stomach,intestine,and add protein inhibitors to investigate the absorption rate constant(Ka),apparent permeability coefficient(Papp)and cumulative absorption of apiin.The effect of exploring the mechanism of absorption of apiin in the body.The results showed that the percentage of apiin absorbed in the stomach was between 4%and 15%,and the absorption of apiin in the stomach was poor.The apiin was absorbed and transported in the small intestine by means of passive transport;after 30,90,150 ?g/mL of apiin was digested and absorbed in the stomach for 2 h,the cumulative absorption was 0.173,0.181 and 0.189 mg,respectively.After 2 h of digestion in the intestine,the cumulative absorption was 0.998,1.089,1.509 mg,respectively.In the small intestine,the absorption size of each intestine is:duodenum>jejunum>ileum>colon.It indicates that the duodenum is the best part of apiin absorption in the small intestine,apiin is a substrate for P-glycoprotein(P-gp)and oligopeptide transporter(PEPT).P-glycoprotein(P-gp)efflux and oligopeptide transporter(PEPT)uptake is celery glycoside The main absorption mechanism of absorption.(2)Using non-target metabolomics techniques to study the metabolic changes of apiin in the process of digestion and absorption in the stomach and intestine.The results showed that there were 22 different metabolites in the positive mode,including 5 phenolic compounds;in the negative mode,a total of 39 metabolites with different metabolites were obtained,including 14 phenolic compounds.The types of different substances mainly include flavonoids,fatty acids,amino acids,sugars,organic acids and the like.The main pathways affecting the absorption and metabolism of apiin are amino acid metabolism,fatty acid metabolism,sugar metabolism and organic acid metabolism.(3)The oxidative damage model of human umbilical vein cells(HUVECs)was used to investigate the antioxidant capacity of apiin metabolites.The results showed that celery glycosides were digested(30,90,150 ug/mL)compared with the model injury group.The NO content of the group increased by 45.88%,67.76%,and 54.58%,respectively.The NO content of the apiin intestine digestion group increased by 19.52%,55.29%,and 28.23%,respectively.The MDA content of the celery glycoside digestion group increased by 43.25%,49.06%and 55.90%respectively.The MDA content of the apiin intestine digestion group increased the apiin by 33.77%,41.25%and 48.25%respectively.The apiin stomach and intestinal metabolites can significantly reduce the intracellular fluorescence intensity,effectively inhibit the production of oxidative free radicals in the cells,have obvious antioxidant capacity,and the antioxidant capacity is positively correlated with the concentration... |
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