Preparation And Performance Study Of Hyaluronic Acid Modified GO@Fe₃O₄ Magnetic Nanocomposite Drug Carrier

As a new type of functional material,magnetic nanomaterials have their unique magnetic properties and surface structure in addition to the commonality of general nanomaterials,which give them more unique functions and properties,and are widely used in many fields especially in the field of biomedicine.Graphene oxide GO is a functional material with large specific surface area,rich functional groups and good biocompatibility.It is easy to modify and suitable to be used as drug carrier.In this paper,GO and Fe₃O₄ were combined into GO@Fe₃O₄ by coprecipitation method.In order to enhance the material stability and the recognition ability of the composite material,the hyaluronic acid HA was modified on the surface of the composite GO@Fe3O4 to prepare a magnetic composite material GO@Fe₃O₄@HA with targeting function.The prepared materials were characterized by UV,IR,TEM,XRD,TGA,magnetic,particle size and potential.The results showed that the magnetic nanocomposite GO@Fe3O4@HA was successfully synthesized.Initial exploration on the drug-loading and drug-releasing properties of the composite material GO@Fe3O4@HA was conducted.The drug loading and the encapsulation rates of GO@Fe₃O₄@HA were 65.56%and 62.98%,respectively.In vitro drug release properties of drug-loaded materials were studied in pH 5.5 PBS and pH 7.4 PBS.At pH 5.5 and pH 7.4,the cumulative release rates of GO@Fe₃O₄@HA were 23.16%and 10.5%,respectively,indicating a certain pH dependence of drug release.The above results show that this magnetic composite material GO@Fe₃O₄@HA has high drug loading and sustained release performance.MTT cytotoxicity experiment was carried out on the prepared magnetic composite material GO@Fe3O4@HA.The results showed that the cell survival rate of GO@Fe₃O₄@HA was 62.83%,the cytotoxicity rating was grade 2 and mild toxicity at the concentration up to 250 ug/ml,which means the composite material has good biocompatibility.Ingestion experiment show that the drug-loaded material can be taken up by Hela cells and can be used as a DOX drug medium with good targeting.