The Application Research Of Electrode-assisted Electrospinning Technology In Laparoscopic Hemostasis And Scald Recovery

Nanomaterials have been widely used in contemporary society.As a method of preparing nanomaterials,electrospinning has the advantages of cheap equipment and easy purchase,easy operation,and no need for special care during the preparation process,which saves manpower.At the same time,due to the barrier,adsorption performance,comfort and shape of the three-dimensional network structure of the material prepared by electrospinning,it has a broad application research status in the field of biomedicine,including: wound dressing,tissue engineering,nerve repair,drug release,artificial blood vessels,etc.However,when materials prepared by electrospinning are used for hemostasis or other wound dressings,an electrospinning film is always prepared first,and then cut into a certain shape on the wound surface.This has certain problems: 1.Unable to perform electrospinning without relying on commercial power;2.Insufficient adhesion of the dressing to the wound surface,may not be able to complete effective hemostasis,or relative friction between the dressing and the wound And secondary injury occurs;3.There is still no quick,effective and non-invasive method of hemostasis in minimally invasive surgery.4.How to promote the slow recovery of deep second degree scald wounds remains to be solved.We used in-situ electrospinning to solve the above problems.First,we combine in situ electrospinning with minimally invasive surgery,which can stably manufacture electrospun nanofibers by laparoscopic and deposit them directly on living organs.In this laparoscopic electrospinning,a tapered convergent structure is also introduced,which brings excellent precision deposition of nanofibers,thereby reducing the deposition area of nanofiber membranes without increasing the fiber diameter.Compared with the traditional hemostasis method,in the minimally invasive surgery of pigs,this laparoscopic electrospinning has the characteristics of fast hemostasis(<5s),less postoperative inflammatory reaction,and quick recovery.This laparoscopic electrospinning provides a new approach to in situ hemostasis in minimally invasive surgery,which can easily be extended to other biomedical applications related to minimally invasive surgery.Second,we synthesized CuS-PDGF-CS polymer nanofibers through a self-made portable electrospinning method.The CuS nanoparticles are prepared by the green method and bovine serum albumin(BSA)step by step.In addition,it should be emphasized that the incorporation of platelet-derived growth factor(PDGF)into these CuS composite nanofibers can accelerate wound healing.Compared with traditional gauze,these CuS-PDGF-CS polymer nanofibers have better densification because they are accurately deposited on the wound in situ using a portable electrospinning device.Under the irradiation of 808 nm infrared light,the expectation of wound disinfection and healing can be achieved simultaneously.The wound healing time was shortened from 30 days to 21 days.This method has attractive application prospects in the field of outdoor scald wound treatment.