The Preparation Of Superparamagnetic Iron Oxide Nanoparticles And The Study Of Magnetic Resonance Imaging Of Brain Glioma

Objective: Superparamagnetic iron oxide nanoparticles(SPIONs)coated with oleylamine were synthesized by the thermal decomposition method,followed by hydrophilic modification with citric acid(CA),lactoferrin was conjugated into surface of SPIONs using EDC method.The ability of Lf-SPIONs enhanced MRI effect in vitro and in vivo was investigated.Methods: Superparamagnetic iron oxide nanoparticles were prepared by the thermal decomposition method.The hydrophobic SPIONs were coated with citric acid by ligand exchange method.Then,Lf was conjugated the surface of SPIONs.The obtained Lf-SPIONs were analyzed by fourier transform infrared(FTIR)spectroscopy.The morphology and hydrodynamic diameters of Lf-SPIONs were observed by Dynamic light scattering(DLS)and vibrating sample magnetometer(VSM).The biocompatibility of Lf-SPIONs was tested by MTT assay,scratch test,hemolysis test,biochemical analysis.The model of rat glioma were established by injected C6 glioma cells and scanned by MR.Results: The FTIR analysis demonstrated the successful conjugation of Lf to the surface of SPIONs,the hydrated particle size was 61.3±4.1 nm,the saturation magnetization was 69 emu/g Fe.The Lf-SPIONs also showed a good biocompatibility in hemolysis,cytotoxicity,cell migration and blood biochemistry studies.In vitro magnetic resonance imaging showed that the signal decreased gradually with increasing the concentration of Lf-SPIONs.The signal intensity of the tumor was significantly reduced after the injection of Lf-SPIONs through tail vein,and turned into a dark signal region,which was obvious difference with the surrounding tissue.Conclusion: Lf was successfully conjugated with SPIONs.The experimental results showed that Lf-SPIONs were good biocompatible.In addition,Lf-SPIONs exhibited good negative contrast enhancement in vitro and in vivo.