The New Type Of Small Molecule Fluorescent Probe Is Used For The Detection Of Nerve Agent Mimics And The Identification Of Urine Protein

The content of this thesis is to design a novel small molecule fluorescent probe for the detection of nerve agent mimics and the recognition of urinary proteins.Nerve agents such as Sarin,Soman,and Tabun are highly toxic organophosphorus?OP?compounds that can be used as chemical weapons.Unfortunately,the ability to quickly detect OP remains an ongoing challenge.Therefore,in Chapter 2,we designed and synthesized a fast-responding fluorescent probe,NAP-P,for the highly selective and sensitive detection of the nerve agent mimic diethyl chlorophosphate?DCP?.The napthalimide fluorophore in this probe acts as a signal motif,and piperazine serves as the reaction site for DCP.The results showed that the fluorescence intensity at 510 nm increased significantly and rapidly?response time<300 s?with the increase of DCP concentration,and the fluorescence quantum yield increased by about 100 times,and the probe has good sensitivity to DCP?LOD=4.5 nM?and high selectivity.Besides,the probe can detect DCP vapor with high selectivity and sensitivity in solution and applied filter paper,showing its potential for practical application.In Chapter 3,we designed a hemicyanine dye as the parent and synthesized the fluorescence-enhanced probe HCY-T and the fluorescence quenching probe HCY-A through Knoevenagel condensation to detect the diethyl cyanophosphonate?DCNP?of tabun gas simulant.The recognition mechanism of the probe is the addition of CN-released by the hydrolysis of DCNP to the indole group of the probe,resulting in the destruction of the probe's conjugated system and the color of the system changed from purple or pink Colorless,accompanied by fluorescence changes;Besides,the two probes are highly selective and sensitive?LOD:probe HCY-T is 4.5 nM,probe HCY-A is 6.4 nM?and fast response?<300 s?.More impressive is that the chemical sensor based on filter paper has obvious naked eye effect on DCNP,thus increasing its potential for practical applicationHuman serum albumin?HSA?is the most abundant thiol-containing transporter in the blood.In addition to promoting the transport of various drugs,fatty acids,and metabolites,it also plays a vital role in maintaining blood osmotic pressure.Generally,HSA concentrations in normal urine and serum are<30 mg L^-1 and³5-55 gL^-1,respectively.However,excessive amounts of HSA in urine are called microalbuminuria,which is an early sign of cardiovascular and kidney disease in patients with diabetes and hypertension.Therefore,in Chapter 4,we developed a BODIPY-based fluorescent probe.By introducing a 3,4-dihydroxyphenyl moiety into the classic structure of BODIPY as a specific recognition site,Highly selective identification of HSA.Besides,the use of the probe BDY-OH allows the quantitative determination of HSA in real urine samples from healthy individuals and urine protein patients.Based on clinical trials,we found that the probe BDY-OH has good proteinuria recognition ability in 40 randomly selected urine samples.All these characteristics show that the probe BDY-OH has practical application prospects in clinical disease pre-diagnosis.