| Cow hoof disease is the third major disease that causes cow elimination.Among them,the incidence of digital dermatitis is as high as 70%.Antibiotic treatment caused the abandonment of milk,resulting in huge economic losses.It is great of significance to improve the therapeutic effect of digital dermatitis and reduce the economic loss to develop non-resistant drugs and improve the method of administration.In this experiment,a salicylic acid ointment was prepared,and a hoof bandage was designed.The therapeutic effect of salicylic acid ointment combined with hoof bandage set on digital dermatitis was studied.The results were compared with that of lincomycin,which provided new drugs,drug delivery methods and research basis for the effective prevention and control of digital dermatitis.Referring to the preparation method of Chinese veterinary pharmacopoeia ointment,salicylic acid ointment was prepared by using dimethyl sulfoxide,propylene glycol and liquid paraffin as solvents and using Vaseline lanolin as matrix.The preparation and stability of soft paste were studied by heat-resistant,cold-resistant and stratified experiments.The results showed that 40% salicylic acid ointment prepared with dimethyl sulfoxide as solvent had good effect,and no stratification and no deterioration and discoloration after long time.According to the incidence of digital dermatitis,the characteristics of drug administration and the anatomical characteristics of hoof,the medical fabric with waterproof,breathable and high elasticity was selected to make the hoof bandage.The clinical application results proved that the hoof bandage was waterproof,easy to administer and ideal to wrap,which could not only isolate feces and urine but also make the medicine fully contact with the affected parts.Randomly selected 40 sick cows,10 g of lincomycin powder mixed with distilled water was applied to the affected area in 20 cows.20 cows were treated with homemade 40% salicylic acid ointment,The two groups were respectively treated with the cloves of cows.Staging was recorded before and after treatment,Score of claudication and skin pain at 0d,3d,14 d and 30 d were recorded.The bacteria were counted before and after treatment.Biopsy samples were taken from the diseased tissue before and after treatment.WarthinStarry staining and HE staining were performed.The effects of two drugs on digital dermatitis were evaluated scientifically.The results showed that after treatment,lameness and skin pain were reduced in both groupscompared with the 0d,with significant difference(P < 0.05),and the difference between the two groups was not significant(P > 0.05).On the third day,there was no recovery in either group,but both the claudication score and the skin pain score were reduced,and the degree of claudication was significantly reduced after treatment.On the 14 th day,the cure rate of salicylic acid was 45% significantly higher than that of the lincomycin group,and the effective rate was75%,which was significantly higher than that of the lincomycin group.On the 30 th day,the improvement rate of the two groups was lower than that at 14 days.The results of bacterial counts before and after treatment showed that the hoof bacteria were significantly reduced after treatment in both groups,and the sterilization rate could reach 99%.The results of W-S staining showed that the number of spirochetes decreased after treatment,the pathological results showed that both drugs could promote the recovery of skin damage in dairy cows.In conclusion,the salicylic acid ointment prepared has a better therapeutic effect on digital dermatitis,and the application effect is more ideal when combined with the development of hoof bandage,providing a research basis for the clinical treatment of digital dermatitis. |
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