| Cow foot disease has become the third largest disease affecting the development of cow industry after mastitis and reproductive system disease.Inter-hoof dermatitis is a common hoof disease in cows.It is manifested as an inflammatory reaction of the skin of the foretoe or hind hoof toe or the plantar side of the foot,redness and swelling between the hoof toe or heel,inflammation and swelling with excrescence.Most cows show normal body temperature and the degree of claudication varies according to the severity.At present,some achievements have been made in the research on the dermatitis between the fingers and toes of cows,including etiological analysis,prevention and control,etc.However,so far,there are still some doubts about the relationship between the microflora and the content of trace elements,so it is extremely urgent to carry out the research of metagenic analysis and trace element detection of the cow’s interphalangeal(toe)dermatitis.In order to investigate interdigital dermatitis in dairy cows,this study conducted PCR detection of Bacteroides nodosa using hoof swabs collected from sick dairy cows in a cattle farm in Qiqihar.The results showed that the cilia gene of bacteroides nodosa was successfully detected,and combined with the clinical symptoms of sick cows in the farm,it suggested that the lame cow on the farm had interdigital dermatitis.Illed cows were treated by washing the hoof,expanding the cutin,removing the submerged channel,soaking the hoof with 5% formaldehyde solution,and copper sulfate.In addition,environmental disinfection work is essential,and pay attention to keeping the hoof clean and dry.In this study,9 samples were collected from a cattle farm in Qiqihar and randomly labeled,(i.e.D group n = 9).At the same time,10 healthy cow foot samples were collected and randomly labeled(i.e.H group n = 10),for the controlled test.Corresponding marker serum sample.Collect hoof swabs of cows whose symptoms have been reduced after 2 weeks of treatment,and label samples randomly(ie T group,n = 10),and the hoof swabs of cows whose symptoms have not been reduced after 2 weeks of treatment,and label samples randomly(ie E group,n = 16);correspondingly collect and label the serum samples of sick cows(group D)and healthy cows(group H).The cilia gene of Bacteroidetes nodule was used for PCR identification,and the diagnosis of cow mesodermatitis was carried out in combination with clinical symptoms.Using the Illumina Mi Seq second-generation sequencing technology based on the variable region of bacterial 16 Sr RNA gene,the bacterial 16 S r RNA gene in the samples was deeply sequenced,and the microbial diversity of the hoof swabs of diseased cows was analyzed through bioinformatics.In this experiment,the content of mineral elements in the serum of sick cows were detected by inductively coupled plasma mass spectrometry(ICP-MS).According to the results of microbial diversity analysis,the content of trace elements in serum of sick cows was further detected in this experiment.The results show that the zap cows suffering from cows(toe)dermatitis,microbial diversity analysis showed that in all samples received 1706485 valid sequences,average sequence length is 442.21 bp,found in the sample of illness high abundance ratios of streptococcus,at the same time,the cow cow serum fingers dermatitis is significantly higher than healthy group,the content of Cu element in Ca element content is significantly higher than that of healthy group,the I element content significantly lower than that of healthy group.In this experiment,the microbial diversity of the hoof part of the cow with the outbreak of digital dermatitis in Qiqihar area was clarified,and the relationship between the main pathogenic bacteria and the content of trace elements was clarified,providing a theoretical basis for the understanding of the pathogenesis of digital dermatitis in cows and the design of relevant prevention and control measures. |
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