Preparation And Performance Study Of Low Swelling Chitosan/silk Fibroin Composite Membrane

Chitosan (CS) as a natural polymer material, with its extensive sources, good biocompatibilityand biodegradable etc, chitosan has been widely used in biomedical fields. But the poormechanical property of chitosan caused by the water absorbing swelling and rapid degradationgreatly limited the application of chitosan. Silk fibroin (SF) is a natural polymer fiber protein,due to its excellent mechanical properties, biocompatibility and good processability, are oftenused in surgical sutures, wound dressings, bone repair and other medical material.At this work, chitson and regeneration silk fibroin were blended to prepare chitosan/silkfibroin (CS/SF) composite films. The relationship between the preparation process and theproperties was studied systematically,,biocompatibility of CS/SF composite films wereinvestigated and prepared CS/SF composite materials with good antibacterial property which hasloaded antibacterial drugs.CS/SF composite films were prepared by solution cast method. The composite films wasanalyzed via Fourier Transform Infrared Spectroscopy (FTIR), density, swelling rate, watervapour transmission rate, tensile strength, elongation at break, degradation rate of compositefilms The result showed that, the structure of chiosan/silk fibroin composite films dominatedby ?-sheet structures; the density of composite films declined with the increaseing of silk fibroincontent; the swelling ratio of composite films was sharply dropped compared with the pure CSfilm, which was dependent on the pH value, when SF content was10%(pH7.4), the swelling ofthe composite films was decreased from143.7%±8.2%to92.7%±3.2%, it showed that the addedof SF protein effectively restrain the swelling ratio of CS film in wet condition. Compared withthe pure CS film, water vapor transmission rate (WVTR) of composite film was also decreasedslightly, which still ranged from2043g/(m2·d) to3363g/(m2·d); The mechanical testing resultsindicated that the hygrometric mechanical properties was improved dramatically which reachedthe maximum when the SF content was15%. The tensile strength of CS/SF composite filmcontaining15%SF were34.5MPa±1.8MPa, which was enhanced by18%compared with thatof pure CS film, while the elongation at break was increased to119.3%±8.2%; the compositefilms have an excellent protection against microbial penetration; STO cells were seeded ontoCS/SF composite films to access cell biocompatibility of the composite films, in vitro experiment indicated that composite films promoted cell adhesion and proliferation. Vancomycinhydrochloride was filled in CS/SF composite films. The films which were placed in PBS solutionto proceed release experiment on swing bed in37?. The drug release testing showed that theCS/SF composite films had controllable release behavior, which exhibited a burst effect in firsthour and subsequently long time controllable release behavior that was better for controllinginfection and accelerating wound healing.To overcome the limitation of pure chitosan film used for medical wound dressing with lowhygrometric mechanical property, chitosan/silk fibroin (CS/SF) composite films were preparedby solution cast method.the molecular interactions between Silk fibroin and chitosan effectivelyimproved the poor mechanical property in the wet environment caused by swelling and rapiddegradation, is expected to be used in biomedical fields.